Phytochemical and Toxicological Properties of Sclerotium from the Edible Fungus - Pleurotus tuber-regium

Chinwe S. Alaribe1*, Mariam Lawal1, Rashidat Momoh1, Ufoma Idaholo1, Pamela Mudabai1, Abdullahi A. Adejare2
1Department of Pharmaceutical Chemistry Faculty of Pharmacy, University of Lagos, Nigeria
 2Department of Physiology, Faculty of Basic Sciences, College of Medicine, University of Lagos, Nigeria

Corresponding Author: [email protected]; [email protected]; Tel: +234-8037263962
Recieved Date: April 20, 2018; Accepted Date: May 09, 2018; Published Date: 10 May 2018
Citation: Alaribe CS, Lawal M, Momoh R, Idaholo U, Mudabai P, Adejare AA. Phytochemical and Toxicological Properties of Sclerotium from the Edible Fungus - Pleurotus tuber-regium. Trop J Nat Prod Res. 2018; 2(5):235-239.  https://doi.org//10.26538/tjnpr/v2i5.6
Copyright: © 2018 Alaribe  et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Edible fungi such as mushrooms have been known to possess great potentials in the production of useful bioactive metabolites for drugs and nutraceuticals. The aim of this study was to investigate the phytochemical constituents present in sclerotium from pleurotus tuber regium and to determine its safety dose using animal model toxicity assay. Phytochemical tests used for preliminary evaluation of the secondary metabolites present in sclerotium was carried out on the n-hexane and 50 % ethanol extracts while the n-hexane extract was subjected to Gas-chromatographic-Mass spectrometric (GC-MS) techniques for the identification of the compounds present. Acute toxicity studies were carried out on aqueous methanol extract of sclerotium using standard in vivo model. Hematological and histopathological parameters were evaluated. Phytochemical analysis results showed the presence of alkaloids, steroids in the n-hexane extract and the presence of cardenolides and steroids in the 50% ethanolextract. GC-MS results showed the presence of nine compounds; Squalene, Dibutyl phthalate, Octadecane, Heneicosane, 9, 17 octacadienal, 2-octyl-cyclopropaneoctanal, carbonic acid, Bis(2-ethylhexyl) phthalate, Nonacosane. The toxicity studies showed no death at the highest dose (5000 mg/kg) administered, although moderate portal and lobular inflammation were observed. The present study has shown that the Sclerotium from the edible fungus - Pleurotus tuber-regium is not toxic to experimental mice at the highest dose of 5000 mg/kg. Hence, the extract is relatively safe when administered orally.

Keywords: Edible Fungus, sclerotium, Pleurotus tuber-regium, GC-MS, toxicity.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr

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