Secondary Metabolites from Curvularia sp, An Endophytic Fungus Isolated from the Leaves of Picralima nitida Durand and Hook (Apocynaceae)

Charles U. Nwachukwu1,2, Kenneth G. Ngwoke2*, Peter M. Eze3, Chuks J. Eboka4, Festus B. C. Okoye2*
1National Agency for Food and Drug Administration and Control, South Eastern Zonal Laboratory, Agulu, Anambra State, Nigeria
2Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria
3Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.
4Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, University of Benin, Edo State, Nigeria

Corresponding Author: [email protected] ; Tel: +2349097627744
Recieved Date: March 05, 2018; Accepted Date: May 05, 2018; Published Date: 10 May 2018
Citation: Nwachukwu CU, Ngwoke KG, Eze PM, Eboka CJ, Okoye FBC. Secondary Metabolites from Curvularia sp, An Endophytic Fungus Isolated from the Leaves of Picralima nitida Durand and Hook (Apocynaceae). Trop J Nat Prod Res. 2018; 2(5):209-213.  https://doi.org//10.26538/tjnpr/v2i5.2
Copyright: © 2018 Nwachukwu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Nature has remained a major source of pharmacologically active compounds used for the treatment of new and existing diseases, or as lead molecules for the development of synthetically derived analogues. This research was carried out to determine the secondary metabolites from the extract of Curvularia sp, an endophytic fungus associated with the leaves of Picralima nitida. The endophytic fungus was isolated and purified from the leaves of the plant material, using the conventional methods. The identity of the fungus was determined by molecular biology characterization. A combination of chromatographic methods (high performance liquid chromatography and column chromatography), spectroscopic techniques (1-D and 2-D nuclear magnetic resonance spectroscopy and mass spectrometry), dereplication and literature data comparison were used to isolate, purify and identify the secondary metabolites in the fermentation extract of the endophytic fungus. Three previously reported compounds, acropyrone (1), 4-hydroxyphenylacetic acid (2) and indole-3-acetic acid (3) were isolated for the first time from Curvularia Sp extract. Compounds 1-3 were tested for microbial and cytotoxic properties. The compounds showed no antimicrobial properties at 1 mg/mL, no observable cytotoxic properties at the dose of 10 µg/mL and no antimycobacterial properties at the dose tested. Although there was no significant bioactivity, the result further confirms the usefulness of endophytic fungi as a secondary metabolite factory for the production of drugs and drug precursors in the laboratory.

Keywords: Endophytic fungus, secondary metabolites, Curvularia sp, structure elucidation, antimicrobial activity.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
ISI Impact Factor: 0.562
Index Copernicus Value (ICV) for 2017: 59.83

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