Prophylactic Effect of Ascorbic Acid On Certain Biochemical and Histopathological Indices In Rats Treated With Repeated Dose of Cyclophosphamide

Tosin O. Olaoba* and Olalekan A. Akinyemi 
Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
Corresponding Author: [email protected]; Tel: +2348065184095
Recieved Date: March 10, 2018; Accepted Date: April 05, 2018; Published Date: 07 April 2018
Citation: Olaoba OT and Akinyemi OA. Prophylactic Effect of Ascorbic Acid on Certain Biochemical and Histopathological Indices In Rats Treated With Repeated Dose of Cyclophosphamide. Trop J Nat Prod Res. 2018; 2(4):185-190.  https://doi.org//10.26538/tjnpr/v2i4.6
Copyright: © 2018 Olaoba and Akinyemi. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

The limited use of cyclophosphamide in cancer treatment resulted from its role in multiple organ toxicities. The present study aimed to investigate the prophylactic capacity of ascorbic acid against hepatotoxicity induced by repeated dose of cyclophosphamide in male rats. Twenty-four male Wistar rats were randomly selected into 4 groups of 6 rats each. Group 1 served as the control and received intraperitoneal injection of normal saline BP 0.9% w/v. Group 2 received freshly prepared cyclophosphamide 4 mg/kg intraperitoneally once daily throughout the period of administration. Group 3 received an oral dose of 100 mg/kg ascorbic acid and then cyclophosphamide while Group 4 received ascorbic acid only. After 21 days of administration, the animals were sacrificed and biochemical estimation as well as histological assessment of the liver were carried out. The result showed significant (P<0.05) decrease in protein concentration, significant (P<0.05) elevation in serum alanine transaminase (ALT), alkaline phosphatase (ALP), gamma glutamyl transpeptidase (GGT) and disrupted liver histological assembly in rats treated with repeated dose of cyclophosphamide as compared to control. Ascorbic acid showed prophylactic capacity against liver damage in cyclophosphamide treated rats. The total protein concentration was normal, the serum liver enzyme activities were not significantly affected and there was protection of the hepatic histoarchitecture when compared with cyclophosphamide treated group. Therefore, the supplementation of ascorbic acid with the use of chemotherapeutic agent such as cyclophosphamide during cancer treatment may prevent related symptoms.

Keywords: Prophylactic, ascorbic acid, biochemical indices, cyclophosphamide.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
ISI Impact Factor: 0.562
Index Copernicus Value (ICV) for 2017: 59.83

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