Brine Shrimp Cytotoxicity and Anti-Mitotic Activity of Aqueous Root-Bark extract of Securidaca longepedunculata (Polygalaceae)

Ridwan A. Lawal1*, Sodiq K. Lawal2, Omolola S. Odesanmi1, Olufemi Isiaq3, Ikeoluwapo O. Adefisan4, Ibrahim A. Badmus1, Jimoh O. Yusuf1, Osaretin A.T. Ebuehi1
1Department of Biochemistry, College of Medicine, University of Lagos, P.M.B 12003, Lagos, Nigeria.
2Department of Human Anatomy, St Francis University College of Health and Allied Sciences, Ifakara, Tanzania.
3Department of Computing, Southampton Solent University, United Kingdom.
4Department of Biological Sciences, College of Basic & Applied Sciences, Mountain Top University, Ogun State, Nigeria.

Corresponding Author: [email protected]; Tel: +234-8056036852
Recieved Date: July 12, 2017; Accepted Date: February 21, 2018; Published Date: 07 March 2018
Citation: Lawal RA, Lawal SK, Odesanmi OS, Isiaq O, Adefisan IO, Badmus IA, Yusuf JO, Ebuehi OAT. Brine Shrimp Cytotoxicity and Anti-Mitotic Activity of Aqueous Root-Bark extract of Securidaca longepedunculata (Polygalaceae). Trop J Nat Prod Res. 2018; 2(3):109-113.  https://doi.org//10.26538/tjnpr/v2i3.1
Copyright: © 2018 Lawal et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Securidaca longepedunculata is a savannah shrub found growing in South-West Nigeria and some other areas in Africa. It has been reported to be used in the treatment of over a hundred ailments. Its root bark is included in several anti-cancer decoctions in Nigeria. Despite its reported use as an anti-cancer plant, there is a dearth of information on the anti-cancer potential of Securidaca longepedunculata in Nigeria. The aim of this research is to determine the cytotoxic and anti-mitotic activity of aqueous root bark extract of Securidaca longepedunculata. Securidaca longepedunculata aqueous root-bark extract was prepared and used for the study. Cytotoxic activity of S. longepedunculata extract was determined by the brine shrimp toxicity assay and Allium cepa assay was used to assess the anti-mitotic activity. Brine shrimp toxicity assay showed an LC50 of 25.1 µg/mL and the Allium cepa assay revealed that 100 mg/mL extract caused a reduction in mitotic index (MI) which was comparable to that of the standard drug, methotrexate. Securidaca longepedunculata has potential as a cytotoxic agent.

Keywords: Allium cepa, Brine Shrimp, Securidaca longepedunculata, cytotoxic, cancer.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr

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