Antioxidative and Free Radical Scavenging Properties of Ethyl Acetate Fractions of Persea americana Seed and Bryophyllum pinnatum Leaf

Emeka S. Asiwe1*, Chidi U. Igwe1, Kizito M.E. Iheanacho1, Ignatius O. Onyeocha2, Viola A. Onwuliri1

1Department of Biochemistry, Federal University of Technology Owerri, Imo State, Nigeria
2Department of Biotechnology, Federal University of Technology Owerri, Imo State, Nigeria


Corresponding Author: [email protected]; Tel: +2348035078005
Recieved Date: 06 June 2021; Accepted Date: 30 July; Published Date: 03 September
Citation: Asiwe ES, Igwe CU, Iheanacho KME, Onyeocha IO, Onwuliri VA. Antioxidative and Free Radical Scavenging Properties of Ethyl Acetate Fractions of Persea americana Seed and Bryophyllum pinnatum Leaf. Trop J Nat Prod Res. 2021; 5(8):1486-1492. http://www.doi.org/10.26538/tjnpr/v5i8.26
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© 2021 Asiwe et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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ABSTRACT

Persea americana and Bryophyllum pinnatum are important plants with age-long application in traditional medicine for the management of varieties of ailments. The seed of P. americana is a byproduct and the leaf of B. pinnatum constituting weed in some locality could be harnessed for marketable antioxidant potentials. The present study aims to assess the antioxidative and free radical scavenging potential of ethyl acetate fractions of Persea americana seed and Bryophyllum pinnatum leaf. This was determined using the effect of the plant fractions on hydroxyl, nitric oxide, and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radicals. Total antioxidant capacity (TAC), reducing power assay and inhibition of H2O2-induced lipid peroxidation in rat stomach homogenates were assessed using standard methods. The extracts exhibited stronger DPPH radicals scavenging effect than tannic acid with threshold inhibitory concentration (IC50) values of 53.10 ± 2.66, 65.59 ± 3.28, and 2039.49 ± 163.16 µg/mL for P. americana, B. pinnatum, and tannic acid respectively. The ethyl acetate fractions exhibited strong hydroxyl and nitric oxide radicals scavenging ability in vitro, with IC50 values of 370.37 ± 14.81, 107.45 ± 5.37, 623.96 ± 31.20 µg/mL and 377.26 ± 18.86, 691.48 ± 48.40,105.86 ± 6.35 µg/mL for P. americana, B. pinnatum, and catechin respectively. Furthermore, the B. pinnatum leaf fraction was found to be highly effective in the inhibition of H2O2-induced lipid peroxidation in rat stomach homogenates. The present study showed that the ethyl acetate fractions of P. americana seed and B. pinnatum leaf possess potent antioxidant properties evidenced in their radical scavenging ability.

Keywords: Oxidative stress, Hydroxyl radical, Nitric oxide, Persea americana, Bryophyllum pinnatum
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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