Friedelin Derivatives from Polygonum flaccidum Showed Cytotoxic Activity on Vero Cell Line

Pritesh R. Dash1, Md. Sohel Rana1, Choudhury M. Hasan2, Md. Abdul Muhit3*
1Department of Pharmacy, Faculty of Biological Sciences, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh
2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh
3Department of Clinical Pharmacy and Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh

Corresponding Author: [email protected] ; Tel: +880-1733-982854
Recieved Date: 09 July; Accepted Date: 26 August ; Published Date: 03 September
Citation: Dash PR, Rana MS, Mahmood Hasan CM, Muhit MA. Friedelin Derivatives from Polygonum flaccidum Showed Cytotoxic Activity on Vero Cell Line. Trop J Nat Prod Res. 2021; 5(8):1456-1459.
© 2021 Dash et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Polygonum flaccidum is an important annual Bangladeshi herb that is being used by the local populace as analgesic, anti-inflammatory and insecticidal agent. In this study, the presence of secondary metabolites in the crude methanol extract of the whole plant of Polygonum flaccidum as well as their cytotoxic activity was investigated. The compounds were isolated using silica gel column chromatography and purified by preparative thin-layer chromatographic technique. Preliminary cytotoxic activity of the column fractions was evaluated through brine shrimp lethality bioassay and toxicity of the isolated compounds were confirmed in Vero cell line.  Three triterpenoids bearing friedelin skeleton in their structures were obtained and were characterized as 19?-hydroxyfriedelin (1), 16?-hydroxyfriedelin (2) and 3?-friedelinol (3) by analyzing their 1D and 2D NMR spectra. Friedelin derivatives are reported for the first time from this plant among which 19?-hydroxyfriedelin (1) has not been previously reported from any natural sources. In the brine shrimp lethality bioassay, LC50 values of the column fractions F-6, F-7 and F-8 were found to be similar to that of positive control (vincristine sulfate). 16?-hydroxyfriedelin (2) and 3?-friedelinol (3) showed potent cytotoxic activity with IC50 value of 8.09 µM/mL and 7.64 µM/mL, respectively in Vero cell line. Therefore, the plant can serve as a potential source of cytotoxic compounds which may be further exploited for new drug development.

Keywords: Polygonum flaccidum, Friedelin derivatives, NMR spectroscopy, Vero Cell line.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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