Time Course Effects of Dietary Vitamin D on Diethylnitrosamine-Induced Oxidative Stress in Rat Kidney

Isaacson B. Adelani1*, Elizabeth O. Ogadi1, Oluwakemi A. Rotimi1, Babatunde M. Duduyemi3, Emmanuel N. Maduagwu2, Solomon O. Rotimi1
1Department of Biochemistry, College of Science and Technology, Covenant University, Ota, Ogun State, Nigeria
2Department of Biochemistry, Chrisland University, Abeokuta, Ogun State, Nigeria
3Department of Pathology, University of Sierra Leone Teaching Hospital Complex: College of Medicine and Allied Health Sciences, Freetown, Sierra Leone

Corresponding Author: [email protected]; Tel: (+234) 7033641899
Recieved Date: 20 March 2021; Accepted Date: 02 June 2021; Published Date: 01 July
Citation: Adelani IB, Ogadi EO, Rotimi OA, Duduyemi BM, Maduagwu EN, Rotimi SO. Time Course Effects of Dietary Vitamin D on Diethylnitrosamine-Induced Oxidative Stress in Rat Kidney. Trop J Nat Prod Res. 2021; 5(6):1118-1124. http://www.doi.org/10.26538/tjnpr/v5i6.23
© 2021 Adelani et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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The kidney is an essential organ known for its role in the endogenous elimination of the bodys waste products. The kidney also functions in metabolic activities, including vitamin D metabolism. Other kidney functions involve transport and reabsorption of solutes exposing the organ to oxidative stress induced by toxicants. The study, therefore, examined the effects of dietary vitamin D (Vit D) on diethylnitrosamine (DEN)-induced oxidative stress in rat kidney. Eighty-four male Wistar rats were divided into four groups of 21 animals each, and 30 mg/kg body weight of DEN was administered twice weekly for 11 weeks. Each group received either DEN + Vit D deficient diet or Vit D diet, and normal saline + Vit D deficient diet, or a Vit D diet. Oxidative stress-antioxidant parameters, including Glutathione S-transferase (GST), Thiobarbituric acid reactive substances (TBARS), Glutathione (GSH), Superoxide dismutase (SOD), and Nitric Oxide (NO), were measured using spectrophotometric methods after weeks 6, 12, and 20. Results showed an early significant (p < 0.05) increase in GST activity with DEN exposure, which was significantly (p < 0.05) reduced with the Vit D diet. In the DEN + Vit D deficient diet group, a significant (p < 0.05) decrease in nephrotic NO concentration and increased SOD activities were observed in the 20th week of evaluation. Nephrotic tubular hyalinization and inflammation were noticeable after 11 weeks of DEN exposure from histopathology results. The findings showed that dietary Vit D could, in part, alleviate kidney oxidative stress effects through oxidative stress modulation.

Keywords: Oxidative stress, Antioxidant, Vitamin D, Diethylnitrosamine, Kidney.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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