Purification and Characterization of Phytase Produced by Aspergillus niger Isolated from Poultry Farm Soil Using Submerged Fermentation 

Adeola E. Onibokun1*, Angela O. Eni1, Solomon U. Oranusi1, Adetutu A. Ajayi2
1Department of Biological Sciences, College of Science and Technology, Covenant University, Ota, Ogun State, Nigeria
2Department of Biological Sciences, Faculty of Science, Augustine University, Epe, Lagos State, Nigeria



Corresponding Author: [email protected]; Tel: +234 803 926 3847
Recieved Date: 28 August 2020; Accepted Date: 25 June 2021; Published Date: 01 July
Citation: Onibokun EA, Eni OA, Oranusi US, Ajayi AA. Purification and Characterization of Phytase Produced by Aspergillus niger Isolated from Poultry Farm Soil Using Submerged Fermentation. Trop J Nat Prod Res. 2021; 5(6):1083-1087. http://www.doi.org/10.26538/tjnpr/v5i6.17
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© 2021 Onibokun et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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ABSTRACT

Phytase is used in feed processing as an exogenous supplement in monogastric animal feed. This study was aimed at determining phytase production capacity of Aspergillus niger isolated from poultry farm soil. A. niger was isolated from poultry farm soil using phytase screening medium (PSM) containing sodium phytate as substrate. Quantitative estimation of phytase production was carried out by submerged fermentation using 3 x 105 spores/100 mL PSM for 5 days at 30oC and 150 rpm. The obtained crude phytase was purified using a combined purification process of ammonium sulphate precipitation and gel filtration chromatography using a 50 x 1.5 cm Sephadex G-75 column. The activity of the partially purified enzyme was assessed by subjecting it to varying pH, temperature, and nutrient conditions. The optimum temperature and pH were 50oC and 6 respectively. The enzyme remained active for over 30 min at 70oC and completely lost activity after 10 min at 80oC. No activity was observed at 100oC. The phytase produced with glucose as carbon source had higher activity (0.087 U/mL) than that produced with sucrose (0.018 U/mL). The A. niger isolate used in this study was unable to utilize ammonium sulphate as a source of nitrogen (no activity). This study establishes the potential of indigenous fungi for industrial phytase production.

Keywords: Phytase, Aspergillus niger, Industry, Submerged fermentation.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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