Characterization of Acid Treated Porcine Mucin and its Effects on Bioadhesion

Sylvester O. Eraga*, Matthew I. Arhewoh, Magnus A. Iwuagwu
Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, University of Benin, Benin City, Edo State, Nigeria.
Corresponding Author: [email protected]; Tel: +234 8030884928
Recieved Date: January 10, 2018; Accepted Date: February 03, 2018; Published Date: 08 February 2018
Citation: Eraga SO, Arhewoh MI, Iwuagwu MA. Characterisation of Acid Treated Porcine Mucin and its Effects on Bioadhesion. Trop J Nat Prod Res. 2018; 2(2):74-79. https://doi.org//10.26538/tjnpr/v2i2.4
Copyright: © 2018 Eraga et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

The study aimed at determining the effect of acid modification on the physicochemical and bioadhesive properties of porcine mucin powder. Mucin powder was treated with solutions of acetic and hydrochloric acids at high and low concentrations and neutralized with equimolar volumes of pyridine and sodium hydroxide solutions, respectively after 24 h and dried. The treated powders were characterized using ultraviolet (UV) scans, solubility tests, pH, melting point, particle size analysis, Differential scanning calorimetry (DSC), Fourier transform infra-red spectroscopy (FTIR), scanning electron microscopy (SEM), powder X-ray diffractometry (PXRD) and bioadhesion measurements. Modified powder UV scans showed a noticeable shift in their peak of absorbances, generally improved solubility, pH range of 4.20 - 4.90, melting point ranging from 105°C to 125°C and mean particle size range from 36.36 ± 6.65 to 114.25 ± 5.65 ?m. Unmodified mucin DSC showed a sharp endothermic transition while those of the modified products were sharp and broad. The melting temperatures corresponded to the endothermic transitions (Tm), ranging from 105°C to 140°C. FTIR showed characteristic change in spectra pattern resulting in broadening of the OH band. PXRD showed distinct and more porous particles with the modified products. Their bioadhesion values ranged from 0.155 to 0.054 Nm-1 with the HCl modified mucin powders showing the highest bioadhesion. Acid modification of native porcine mucin powders resulted in the formation of modified products with superior bioadhesive properties over the native porcine mucin powders. These modified products have a potential application in bioadhesive drug delivery systems.

Keywords: Mucin, Acid treatment, Modified, Powder, Analyses, Bioadhesion.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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