Glycoprotein Isolated from Eurycoma longifolia (Tongkat Ali) is Capable of Boosting Testosterone Levels in Leydig cells

Jaya Vejayan1*, Yasmin A.C. Yahya1, Sharifah A.T. Said1, Aini Norhidayah1, Venugopal Jayarama1, Normaiza Zamri1, Halijah Ibrahim2
1Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang. Lebuhraya Tun Razak, Pahang, 26300 Kuantan, Malaysia
2Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia

Corresponding Author: [email protected]; Tel: +6016-6063804
Recieved Date: 18 May 2021; Accepted Date: 27 June 2021; Published Date: 01 July
Citation: Vejayan J, Yahya YAC, Said SAT, Norhidayah A, Jayarama V, Zamri N, Ibrahim H. Glycoprotein Isolated from Eurycoma longifolia (Tongkat Ali) is Capable of Boosting Testosterone Levels in Leydig Cells. Trop J Nat Prod Res. 2021; 5(6):1078-1082.
© 2021 Vejayan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Visit for more related articles at  Tropical Journal of Natural Product Research


Eurycoma longifolia (Tongkat Ali) is renowned for its aphrodisiac potential, and its active constituent has been presumed to be a protein and more likely a glycosylated protein. In this study, the ability of the glycoprotein to increase testosterone hormone levels was investigated. The dried root powder of the plant was extracted using water under reflux. The protein fraction was separated using size-exclusion chromatography and subjected to SDS-PAGE analysis. Thereafter the protein fraction was isolated from its glycoprotein using a lectin column. Finally, TM-3 Leydig cells were treated with the isolated glycoprotein fraction (50 µg/mL). The extraction yielded 14.3% w/w protein and the SDS PAGE analysis showed a single band at approximately 20 kDa. Treatment of TM-3 Leydig cells with the glycoprotein fraction for 72 hours demonstrated an increase in testosterone levels by almost 100% (0.36 ± 0.03 nmol/L) in comparison to the untreated cells (0.18 ± 0.05 nmol/L). The findings suggested that the glycoprotein in E. longifolia root can be easily isolated because its sugar moiety can bind to a lectin affinity column. Moreover, this glycoprotein was shown to have testosterone-boosting activity. These findings identified the glycoprotein as the bioactive constituent associated with its aphrodisiac properties.

Keywords: Protein, Leydig cells, Testosterone, Aphrodisiac.
Back to Articles

ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
Index Copernicus Value (ICV) for 2017: 59.83
Scopus citescore 0.3 (2020)

Indexing & Abstracting

citescore 0.3 (2020)

j-gate logo

International Innovative Journal Impact Factor

African Index Medicus


Index Copernicus International

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License

Crossref Content Registration logo

WorldCat Discovery Service

Geneva Foundation for Medical Education and Research