Membrane Stabilizing Effect and Antioxidant Potential of Different Extracts from Eicchornia crassipes Flowers: An In Vitro Assessment

Prasanth M. Kumar1*, Venkatesan Suba2, Rami B. Reddy3, Srinivasa P. Babu1
1Vignan Pharmacy College, Vadlamudi, Andhrapradesh, India.
2Department of Pharmacology, National Institute of Siddha, Chennai, India.
3Director-Formulations, NATCO Pharma, Telangana, India.

Corresponding Author: [email protected]; Tel: +91-9985787082
Recieved Date: November 29, 2017; Accepted Date: December 27, 2017; Published Date: 07 January 2018
Citation: Kumar PM, Suba V, Reddy RB, Babu SP. Membrane Stabilizing Effect and Antioxidant Potential of Different Extracts from Eicchornia crassipes Flowers: An In Vitro Assessment. Trop J Nat Prod Res. 2018; 2(1):23-28.
Copyright: © 2018 Kumar et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Eicchornia crassipes has been traditionally used as a tonic for the skin of horses, for irritation and inflammation. The aim of this study was to evaluate the total phenolic and flavonoid contents, antioxidant and membrane stabilizing activities of different extracts from E. crassipes. In vitro antioxidant activity was determined by DPPH radical scavenging, ABTS radical scavenging, hydroxyl radical scavenging and reducing power assays. The total flavonoid and phenolic contents were also investigated. Membrane stabilizing potential of the extracts was studied on human erythrocytes in hypotonic solution and by heat-induced hemolysis procedures. Quantitative analysis revealed the highest amount of total phenols and flavonoids in the ethanol extract while the lowest amount was in the ethyl acetate extract. Ethanol extract exhibited promising antioxidant potential in the DPPH, ABTS, and hydroxyl radical scavenging assays compared to the other extracts. Also, the ethanol extract possesses strong reducing power ability compared to the other extracts. A significant correlation was found between the antioxidant activity of the extracts and their total phenolic and total flavonoid contents. The ethanol extract exhibited the highest percentage hemolysis inhibition in hypotonic solution-induced hemolysis and in heat-induced hemolysis procedures. Results from this study showed that the flowers of E. crassipes exhibited in vitro antioxidant and anti-inflammatory activities. This study substantiated the folkloric use of E. crassipes flowers in inflammatory disorders.

Keywords: Eicchornia crassipes, antioxidant, anti-inflammatory, flavonoids, phenolic compounds, in vitro assay
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