Differentiation of Oil Palm Hybrid from Parentals Using As-PCR Marker

Vona D. Odenore1, Geoffrey O. Anoliefo2, Omorefe Asemota1, Beckley Ikhajiagbe2*, Leonard O. Ihase1, Chukwuemeka R. Eke1
1Nigerian Institute for Oil Palm Research, Benin City, Nigeria. 2Environmental Biotechnology and Sustainability Research Group, Dept. of Plant Biol. and Biotechnology, University of Benin, Benin City
Corresponding Author: [email protected]; Tel: +2348037094470
Recieved Date: October 04, 2017; Accepted Date: November 22, 2017; Published Date: 05 December 2017
Citation: Odenore VD, Anoliefo GO, Omorefe A, Ikhajiagbe B, Ihase LO, Eke CR. Differentiation of Oil Palm Hybrid from Parentals Using As-PCR Marker. Trop J Nat Prod Res. 2017; 1(6):259-261. https://doi.org//10.26538/tjnpr/v1i6.7
Copyright: © 2017 Odenore et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Oil palm (Elaesis guineensis) is a highly economic oil producing tree that produces fruits of three forms which cannot be differentiated until reproductively matured. The commercially acceptable form is the Tenera fruit form because of its high oil-yielding capacity. It is a hybrid of Dura and Pisifera. The aim of this study was to determine alleles unique to Tenera fruit form of the Nigerian oil palm. Allele Specific Polymerase Chain Reaction (AS-PCR) method was used in this study. It required DNA isolation, common shell gene primer testing, allele specific primer pair investigation as right and left primers and a multiplex PCR investigation consisting of common and allele specific primers for the shell gene. These primer combinations were used to screen the genome of Dura, Pisifera and Tenera for variants in the shell gene. The findings from this study suggest two alleles of sizes 400 bp and 200 bp unique to Tenera when the primer combinations S3F&R and S22&S32 was used. This can serve as a marker for differentiating Tenera from Dura and Pisifera. The outcome of this research will aid early detection of choice variety for distribution to Oil palm growers.

Keywords: Oil palm, Tenera, Dura, Pisifera, Allele Specific-PCR.
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