High Performance Liquid Chromatography Profiling of Dennettia tripetala Leaf Extract and Its Biological Activities



  • Dilibe C. Urama Plant Science and Biotechnology Department, University of Nigeria, Nsuuka, Nigeria
  • Kingsley O. Omeje Department of Biochemistry, University of Nigeria, Nsuuka, Nigeria
  • Benjamin O. Ezema Department of Biochemistry, University of Nigeria, Nsuuka, Nigeria
  • Juliet N. Ozioko epartment of Biochemistry, University of Nigeria, Nsuuka, Nigeria
  • Henry C. Omeje Department of Biochemistry, University of Port Harcourt, Choba, Nigeria
  • Ugwu Ezinne Department of Biochemistry, University of Nigeria, Nsuuka, Nigeria


Dennttia tripetala, Phytochemistry, Antioxidant, Anti-cholinesterases, HPLC


There are much potential embedded in plants, ranging from air purification to ornamental and source of medicine to mankind. This study reports the biological potentials of Dennettia tripetala leaf extract and its flavonoid content for its possible usage in ameliorating. Flavonoid content of D. tripetala leaf was profiled using high performance liquid chromatography (HPLC), antioxidant and anticholinesterase properties were also studied using standard methods. The major flavonoids identified and quantified were catechin (56.17 mg/100g), kaemferol (49.80 mg/100g), quercetin (69.19 mg/100g), isorhammnetin (19.89 mg/100g) and naringin (19.55
mg/100g). There was evidence of antioxidant potential of D. tripetala leaf extract. Also, the compound, flavonoid, showed acetylcholinesterase and butrylcholinesterase inhibition of 68 and 74%, respectively. The results of the study indicated that the leaf extract of D. tripetala contains high concentrations of flavonoids and possesses antioxidant and anticholinesterases properties. It
may be used in managing oxidative stress-related diseases and dementias. 


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How to Cite

Urama, D. C., Omeje, K. O., Ezema, B. O., Ozioko, J. N., Omeje, H. C., & Ezinne, U. (2020). High Performance Liquid Chromatography Profiling of Dennettia tripetala Leaf Extract and Its Biological Activities: doi.org/10.26538/tjnpr/v4i11.26. Tropical Journal of Natural Product Research (TJNPR), 4(11), 995–999. Retrieved from https://www.tjnpr.org/index.php/home/article/view/971