Screening and Optimization of Culture Conditions for Local Production of Tannase from Fungal Species

doi.org/10.26538/tjnpr/v4i12.25

Authors

  • Toluwanimi V. Afolayan Department of Biotechnology, FIIRO, PMB 21023, Ikeja, Lagos, Nigeria
  • Moses E. Adah Department of Biotechnology, FIIRO, PMB 21023, Ikeja, Lagos, Nigeria
  • Olubunmi I. Ibidapo Department of Biotechnology, FIIRO, PMB 21023, Ikeja, Lagos, Nigeria
  • Moses O. Orotope Department of Biotechnology, FIIRO, PMB 21023, Ikeja, Lagos, Nigeria
  • Frank A. Orji Department of Biotechnology, FIIRO, PMB 21023, Ikeja, Lagos, Nigeria
  • Ariyo C. Famotemi Department of Biotechnology, FIIRO, PMB 21023, Ikeja, Lagos, Nigeria
  • Joy Ehiwuogu-Onyibe Department of Biotechnology, FIIRO, PMB 21023, Ikeja, Lagos, Nigeria
  • Adekunle K. Lawal Department of Biotechnology, FIIRO, PMB 21023, Ikeja, Lagos, Nigeria

Keywords:

Tannase, Fermentation, Culture conditions, Optimum temperature, Tannin, Optimum pH

Abstract

Tannase is produced extensively from microorganisms such as fungi, bacteria and yeast as they are diverse naturally. The aim of this study was to isolate, screen and select local microbial strain for the production of tannase as well as optimize culture conditions for the hyper producers. Fifteen fungal species were isolated from decomposed tannin rich materials (soil under Almond leaves undergoing natural deterioration) and its surrounding soil. They were screened with mineral medium compounded with tannic acid. Selected isolates were used for tannase production under solid state fermentation and varied the fermentation conditions. All strains showed various degree of tannic acid utilization as carbon source for enzyme (tannase) production. Three isolates were identified to be potential hyper producers. The fungal species; Aspergillus niger, Penicillum sp. and Aspergillus flavipes yielded the highest enzyme production after incubation. Optimum temperature and pH for tannase production was recorded as 28-45°C and 5.5 respectively. The isolates were good tannase producer with high activities of the enzyme, especially Aspergillus niger, Penicillum sp. and Aspergillus flavipes which gave a high yield of tannase. 

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Published

2020-12-01

How to Cite

Afolayan, T. V., Adah, M. E., Ibidapo, O. I., Orotope, M. O., Orji, F. A., Famotemi, A. C., … Lawal, A. K. (2020). Screening and Optimization of Culture Conditions for Local Production of Tannase from Fungal Species: doi.org/10.26538/tjnpr/v4i12.25. Tropical Journal of Natural Product Research (TJNPR), 4(12), 1178–1181. Retrieved from https://www.tjnpr.org/index.php/home/article/view/919