Investigation of the Antidiabetic Activity and GC-MS Analysis of Extracts of Lilium polyphyllum


  • Amin M. Mir Assistant Research Professor, Department of Mathematics & Natural Sciences, Prince Mohammad Bin Fahd University, AlKhobar, Saudi Arabia
  • Muhammad W. Ashraf Dean Department of Mathematics & Natural Sciences, Prince Mohammad Bin Fahd University, AlKhobar, Saudi Arabia
  • Swarn P. Singh Research Scholar, IIIM Jammu (Jammu and Kashmir) India
  • Shailendra P. Madwal Professor Dept. of Chemistry, Govt. (PG) College Jaiharikhal Pauri Garhwal (Uttarakhand) India
  • Bilal A. Mir Assistant Professor, Department of Environmental Sciences & Microbiology UCBMSH Dehradun Uttarakhand India


Alpha glycosidase, Alpha amylase, Lilium polyphyllum, Antidiabetic


Diabetes is a disease characterized by high blood glucose (hyperglycaemia) due to insufficient insulin production or impaired tissue response to insulin. The hormone Insulin, produced in the pancreas, helps in the regulation of blood glucose. The present study is aimed at finding an alternative approach to blood glucose regulation by the use of Lilium polyphyllum Stem extract as potential inhibitor of the enzymes; α-amylase and α-glycosidase, which are responsible for the breakdown of oligosaccharides into glucose molecules. The α-amylase and α-glycosidase inhibitory activities of the extracts of Lilium polyphyllum in vitro using standard procedures. The ethanol extract of the plant was subjected to gas chromatographic-mass spectrometric (GC-MS) analysis. The results showed inhibition of α-amylase by 54.95% (water extract), 53.01% (ethanol extract) and 47.87% (dichloromethane extract). The inhibition of α-glucosidase was found to be 48.48%, 48.48% and 43.85% for water, ethanol and dichloromethane extracts, respectively. The possible compounds that could be responsible for the enzyme inhibition were identified by GC-MS analysis as; Methyl 2-furoate, 5-hydroxymethyl furfural, Piperine, Palmitic acid, and Methyl Palmitate. The results revealed that the extracts of Lilium polyphyllum could be a possible source of natural antidiabetic medicine.


Merlin T, Con T, Richard M, George J. Anaemia in diabetes: An emerging complication of micro vascular disease. Curr Diab Rev. 2005; 1:107–126.

Sunday Times India, Diabetes now a global threat gets own day. Sunday Times India 2006; 24:11.

Pepato MT, Mori DM, Baviera AM, Harami JB, Vendramini RC, Brunetti IL, Fruit of the Jambolan tree (Eugenia jambolana Lam.) and experimental diabetes. J Ethnopharmacol. 2005; 96:43–48.

Venkatesh S, Reddy GD, Reddy BM, Ramesh M, Apparao AVN, Antihyperglycemic activity of Carulluma attenuate. Fitoterapia. 2003; 74:274–277.

Sharma BD and Balkrishna AV. Vitality strengthening Astavarga Plants (Jeevaniya & Vayasthapan Paudhe). Divya Publishers, Divya Yog Mandir, Haridwar. Uttaranchal. 2005; 20-80.

Das AK and Rai A world without diabetes and its complications: A preventive program. In: Jayaram BM (Ed.), Type 2 Diabetes and its complications (A preventive program. Microlabe limited. Bangalore. 2008; 1-2 p.

Gupta R and Misra A. Type 2 diabetes in India: regional disparities. Br J Diab Vasc Dis. 2007; 7:12-16.

Sharma AR, Sushruta Samhita (Part-1). Varanasi, India: Chaukhambha Sankrit Pratisthan. 2012. 10 p.

Pandeya G, Sashtri K, Charaka Samhita, (part I) (SanskritHindi) Chaukhambha Bharatee Academy, Varanasi, India. 2011; 587 p.

Sunday Times India, Diabetes now a global threat gets own day. Sunday Times India 2006; 24:11.

Sharma I and Singh A. Clinical Studies with Ayurvedic Formulations-A Practitioner’s Preview. J Nat Ayurvedic Med. 2017; 1(1):1-5.

Subramoniam A, Madhavachandran V, Gangaprasad A. Medicinal plants in the treatment of arthritis. Ann Phytomed. 2013; 2(1):3-36.

Ayurvedic Pharmacopoeia of India. Ministry of Health and Family Welfare Department of Indian System of Medicine and Homeopathy, New Delhi. 2001; 5:103.

Dhyani A, Bahuguna YM, Semwal DP, Nautiyal BP, Nautiyal MC. Anatomical features of Lilium polyphyllum D. Don ex Royle (Liliaceae). J Am Sci. 2009; 5(5):85-90.

Dhyani A. Lilium polyphyllum-rarest of rare lilies. Nordic J Bot. 2010; 30(4):85-91.

Kumari S. Pharmacognostical & Phytochemical studies of Roscea precera (Kakoi) and Lilium polyphyllum (Ksheerkakoli) in comparison with market samples. Pharmacogn J. 2011; 3(25):32-38.

Chang C, Chen CT, Tsai YC, Chang WC. A tissue culture protocol for propagation of a rare plant, Lilium speciosum Thunb. var. glorisoides Baker. Bot Bull Acad Sin. 2000; 41:139-142.

Abate N and Chandalia M. Ethnicity, type 2 diabetes & migrant Asian Indians. Ind J Med Res. 2007; 125:251-258.

Hamdan II and Afifi FU. Studies on the in vitro and in vivo hypoglycemic activities of some medicinal plants used in treatment of diabetes in Jordanian traditional medicine. J Ethnopharmacol. 2004; 93:117-121.

Heidari R, Zareae S, Heidarizadeh M. Extraction, Purification, and Inhibitory Effect of Alpha-Amylase Inhibitor from Wheat (Triticum aestivum Var. Zarrin). Pak J Nutr. 2005; 4(2):101-105.

Krishnaveni S, Theymoli B, Sadasivam S. Phenol Sulphuric Acid method. Food Chem. 1984; 15:229.

Shai LJ, Masoko P, PMokgotho M. Yeast alpha glucosidase inhibitory and antioxidant activities of six medicinal plants collected in Phalaborwa, South Africa. South Afr J Bot. 2010; 76:465 470.

Hema R, Kumaravel S, Gomathi S, Sivasubramaniam C. Gas Chromatography-Mass Spectroscopic analysis of Lawsonia inermis leaves. New York Sci J. 2010; 3:141-143.

Rajiv GR and Sasikumar GP. Antidiabetic effect of Merremia emarginata Burm.F in Streptozotocin induced diabetic rats. Asian Pac J Trop Biomed. 2012; 2: 281-286.

Rhabaso LR. and Chiasson JL, Glucosidase inhibitors. In: Defronzo R.A, Ferrannini E, Keen H, Zimmet P. (Eds.), International Textbook of Diabetes Mellitus, Vol. 1, third ed. John Wiley and Sons Ltd. UK. 2004. 901-914 p.

Davis SN and Granner DK. Insulin, oral hypoglycemic agents and the pharmacology of endocrine pancreas. In: Brunton LL, Lazo JS, Parker KL (Ed.), Goodman and Gilman’s: The pharmacological basis of therapeutics, 11th ed. (McGraw-Hill Medical Publication Division: New York. 2001. 1706-1707 p.

Conforti F, Statti G, Loizzo MR, Sacchetti G, Poli F, Menichini F. Traditional Medicinal Herbs and Food Plants Have the Potential to Inhibit Key Carbohydrate Hydrolyzing Enzymes In Vitro and Reduce Postprandial Blood Glucose Peaks In Vivo. Bio Pharm Bull. 2005; 28(6):1098-1102.

Xu Z, Wang X, Zhou M, Ma L, Deng Y, Zhang H, Zhao A, Zhang Y, Jia W. The antidiabtic activity of total lignan from Fructus arctii against alloxan-induced diabetes in mice and rats. Phytother Res. 2008; 22:97–101.

Lenzen S. The mechanisms of alloxan- and streptozotocininduced diabetes. Diabetolog. 2008; 51:216–226.

Ghosh R, Sharatchandra KH, Rita S, Thokchom IS. Hypoglycemic activity of Ficus hispida (bark) in normal and diabetic albino rats. Indian J Pharmacol. 2004; 36:222– 225.

Matsui T, Ueda T, Oki T, Sugita K, Terahara N, Matsumoto K. α-Glucosidase inhibitory action of natural acylated anthocyanins. J Agric Food Chem. 2001; 49:1948–1951.

Kumar GPS, Arulselvan P, Kumar DS, Subramanian P. Antidiabetic activity of fruits of Terminalia chebula on streptozotocin-induced diabetic rats. J Health Sci. 2006; 52(3):283–291




How to Cite

Mir, A. M., Ashraf, M. W., Singh, S. P., Madwal, S. P., & Mir, B. A. (2020). Investigation of the Antidiabetic Activity and GC-MS Analysis of Extracts of Lilium polyphyllum: Tropical Journal of Natural Product Research (TJNPR), 4(12), 1190–1195. Retrieved from