Chemical Composition, Antioxidant Potential and Antimicrobial Properties of the Essential Oils of Haplophyllum tuberculatum (Forsskal) A. Juss from Morocco

doi.org/10.26538/tjnpr/v4i12.13

Authors

  • Abdelkrim Agour Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health & Quality of Life, Department of Biology, Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez, Morocco
  • Ibrahim Mssillou Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health & Quality of Life, Department of Biology, Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez, Morocco
  • Hamza Saghrouchni Laboratory of Biotechnology, Environment, Agri-food and Health, Department of Biology, Faculty of Sciences, Sidi Mohamed Ben Abdellah and University, Fez, Morocco
  • Amina Bari Laboratory of Biotechnology, Environment, Agri-food and Health, Department of Biology, Faculty of Sciences, Sidi Mohamed Ben Abdellah and University, Fez, Morocco
  • Badiaa Lyoussi Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health & Quality of Life, Department of Biology, Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez, Morocco
  • Elhoussine Derwich Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health & Quality of Life, Department of Biology, Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez, Morocco

Keywords:

Antimicrobial activity, Antioxidant, GC/MS, Essential oils, H. tuberculatum

Abstract

Essential oils are secondary metabolites of plants which have shown very important biological activities. The chemical composition, antioxidant and antimicrobial activity of essential oils isolated from Haplophyllum tuberculatum (Forsskal) A. Juss are the subject of this study. The essential oils was extracted by hydrodistillation, GC/MS and GC-FID were used to determine the chemical composition of the essential oils studied. Two tests were used to assess antioxidant power DPPH (2,2-diphenyl-1-picrilhidrazil) test and TAC (Total antioxidant capacity) assays. Agar diffusion and microdilution were used to examine the antimicrobial activity of these oils. The GC/MS and GC-FID analysis reveal 50 components. The major component was Farnesyl
bromide (37.02%) followed by santolina triene (10.26%), terpinene-4-ol (6.13%) and limonene (2.26%). The scavenging capacity of DPPH by essential oils (IC50 = 3.2395 ± 0.009 mg/mL) was lower than that of ascorbic acid (IC50= 2.8195 ± 0.008 μg/mL), but the measurement of its TAC revealed the presence of a significant amount of antioxidants in the oils studied (617.95 ± 0.589 mg EAA/g extract). S. aureus (bacteria G +) appears the most sensitive (24 mm). The values of the MIC are proportional to the values of the diameters of the inhibition zone, the oil was very active on S. aureus (MIC = 5.91μg/mL). E. coli and P. aeruginosa were the least sensitive (MIC = 94.6 μg/mL). The results of this study showed that this oil can be an alternative source of antimicrobials to combat strains with a problem of resistance to conventional antibiotics.

Author Biography

Elhoussine Derwich, Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health & Quality of Life, Department of Biology, Faculty of Sciences, Sidi Mohamed Ben Abdellah University, Fez, Morocco

Unity of GC/MS and GC, City of Innovation, Sidi Mohamed Ben Abdellah University, Fez, Morocco

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Published

2020-12-01

How to Cite

Agour, A., Mssillou, I., Saghrouchni, H., Bari, A., Lyoussi, B., & Derwich, E. (2020). Chemical Composition, Antioxidant Potential and Antimicrobial Properties of the Essential Oils of Haplophyllum tuberculatum (Forsskal) A. Juss from Morocco: doi.org/10.26538/tjnpr/v4i12.13. Tropical Journal of Natural Product Research (TJNPR), 4(12), 1108–1115. Retrieved from https://www.tjnpr.org/index.php/home/article/view/888

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