Antibacterial Activity and Stability Evaluation of “Apo-taat” Remedy Extract for Inhibiting Diarrhoea-Causing Bacteria

doi.org/10.26538/tjnpr/v4i12.12

Authors

  • Sumalee Panthong Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand
  • Intouch Sakpakdeejaroen Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand
  • Pranporn Kuropakornpong Student of Doctor of Philosophy in Medical Science Program, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand
  • Jaiboonya Jaicharoensub Student of Master of Science Program in Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand
  • Arunporn Itharat Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand

Keywords:

Apo-taat remedy, Anti-bacterial activity, Caesalpinia sappan, Stability testing, Phyllanthus emblica

Abstract

Apo-taat, a plant-based, Thai traditional medicine that contains Caesalpinia sappan and Phyllanthus emblica, is used for treating diarrhea. In the present study, the ingredients of the Apo-taat remedy were extracted, analysed and investigated for antibacterial activity and stability testing under forced degradation and accelerated conditions. Apo-taat extract showed good antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serovar Typhi, Shigella dysenteriae, Staphylococcus aureus, methicillin-resistant S. aureus and Bacillus subtilis with MIC values of 0.313 to 1.25 mg/mL. The main chemical constituent of Apo-taat extract was brazilin (51.94 mg/g of dried extract) and gallic acid (7.30 mg/g of dried extract). Brazilin and gallic acid were unstable under acid and alkaline conditions but stable under oxidation and thermal degradation and over six months. These results can be concluded that Apo-taat showed good antibacterial activity and stable under oxidation condition, thermal condition and long-term storage at room temperature.

Author Biographies

Sumalee Panthong, Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand

Centre of Excellence in Applied Thai Traditional Medicine Research (CEATMR), Thammasat University, Pathumthani 12120, Thailand

Arunporn Itharat, Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand

Centre of Excellence in Applied Thai Traditional Medicine Research (CEATMR), Thammasat University, Pathumthani 12120, Thailand

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Published

2020-12-01

How to Cite

Panthong, S., Sakpakdeejaroen, I., Kuropakornpong, P., Jaicharoensub, J., & Itharat, A. (2020). Antibacterial Activity and Stability Evaluation of “Apo-taat” Remedy Extract for Inhibiting Diarrhoea-Causing Bacteria: doi.org/10.26538/tjnpr/v4i12.12. Tropical Journal of Natural Product Research (TJNPR), 4(12), 1101–1107. Retrieved from https://www.tjnpr.org/index.php/home/article/view/887