In-silico and In-vitro Studies of Antioxidant and Sun Protection Activities of Sappan Wood (Caesalpinia sappan L.)

doi.org/10.26538/tjnpr/v4i12.8

Authors

  • Ni Putu L. Laksmiani Department of Pharmacy, Mathematics and Natural Science Faculty, Udayana University, Bukit Jimbaran 80361, Bali, Indonesia
  • Ni Putu E. Leliqia Department of Pharmacy, Mathematics and Natural Science Faculty, Udayana University, Bukit Jimbaran 80361, Bali, Indonesia
  • Pande M. N. Armita Department of Pharmacy, Mathematics and Natural Science Faculty, Udayana University, Bukit Jimbaran 80361, Bali, Indonesia
  • Nyoman I. G. K. Arijana Department of Histology, Faculty of Medicine, Udayana University, Sudirman 80234, Bali Indonesia
  • Anak A. B. Y. Saputra Department of Pharmacy, Mathematics and Natural Science Faculty, Udayana University, Bukit Jimbaran 80361, Bali, Indonesia
  • Kadek I.  Prananingtyas Department of Pharmacy, Mathematics and Natural Science Faculty, Udayana University, Bukit Jimbaran 80361, Bali, Indonesia

Keywords:

Antioxidant, Docking, DPPH, FIC, Sappan wood, Sun protection

Abstract

Sappan wood which contains brazilin and brazilein is known to have antioxidants potential because of its hydroxyl groups. The use of antioxidants in sunscreen preparations increases photoprotective activity. The purpose of this study was to evaluate the antioxidant activity of sappan wood, to determine the affinity of brazilein and brazilin for glutathione peroxidase (GPX) and catalase protein targets in silico, and describe the ability of sappan wood as an in vitro sun protection agent. The
antioxidant activity was assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and ferrous ion chelating (FIC) assays, while the in silico test was carried out by using molecular docking with the autodock 4.2 programs. Sunscreen activity was tested by determining the sun protection factor (SPF) using the spectrophotometric method. Sappan wood was found to have very high antioxidant activity, with IC50 values of 0.88 ppm and 1.75 ppm for the ethyl acetate fraction and
methanol extract, respectively, which were lower than that of ascorbic acid (7.8 ppm) in the DPPH scavenging assay.. In the FIC assay, the IC50 values were 69.46, 62.59, and 10.21 ppm for the extract, the fraction and EDTA, respectively. SPF value of the extract and the fraction of sappan wood were 17.91 and 19.53 at a 200 ppm concentration each. Brazilein and brazilin in sappan wood can induce GPX and catalase. Sappan wood has great potential to be developed as a sunscreen with a dual
function as antioxidants and as UVB rays absorber. 

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Published

2020-12-01

How to Cite

Laksmiani, N. P. L., Leliqia, N. P. E., Armita, P. M. N., Arijana, N. I. G. K., Saputra, A. A. B. Y., & Prananingtyas, K. I. (2020). In-silico and In-vitro Studies of Antioxidant and Sun Protection Activities of Sappan Wood (Caesalpinia sappan L.): doi.org/10.26538/tjnpr/v4i12.8. Tropical Journal of Natural Product Research (TJNPR), 4(12). Retrieved from https://www.tjnpr.org/index.php/home/article/view/860