Unveiling Nature's Treasure: Investigating the Antioxidant and Anticancer Activities of Mas Banana (<i>Musa acuminata colla</i>) Bracts from Lampung, Indonesia
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Abstract
Over the past decade, there has been a global focus on herbal medicines derived from plants. Mas Banana bracts have great potential to be transformed into natural medicine and nutraceuticals to improve the global health, in addition to being a local food supply. This study explores the phytochemical content, antioxidant, and anticancer activity of Mas Banana bracts grown in Lampung, Indonesia. Mas Banana bracts were fractionated with ethyl acetate. Using LC-MS/MS QTOF (Liquid Chromatography-Mass Spectrometry/Tandem Mass Spectrometry Quadrupole Time-of-Flight), the phytochemical composition of the ethyl acetate fraction of mas banana bracts
was investigated. The analysis revealed the presence of Afzelechin and Moracenin D. The antioxidant potential was assessed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, yielding a value of 810.79 mg/100 g. The anticancer activity tested against the HeLa cell line using standard procedures was seen from the cells viability percentage that reached 51.28%. The ethyl acetate fraction of Mas Banana bracts exhibited significant antioxidant and anticancer activities with greater activity against the HeLa cell line. The results show that the ethyl acetate fraction of Mas Banana bracts possesses anticancer and antioxidant properties and it can be used as a natural food preservative and as an anticancer agent for the treatment of cancer.
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References
Dewi S, Ahmad F, Khastini RO, Leksono SM, Maryani N. Bananas and Their Wild Relatives in Pandeglang, Indonesia. J Biosci. 2023;30(6):1071-1091.
Kusuma HS, Diwiyanto YM, Jaya DEC, Amenaghawon AN, Darmokoesoemo H. Evaluation of drying kinetics, electric and emission study of Musa paradisiaca L. leaves using microwave-assisted drying method. Appl Food Res. 2023; 3(2):1-10.
Maryani N, Lombard L, Poerba YS, Subandiyah S, Crous PW, Kema GHJ. Phylogeny and genetic diversity of the banana Fusarium wilt pathogen Fusarium oxysporum f. sp. cubense in the Indonesian centre of origin. Stud Mycol. 2019; 92(1):155-194.
Statistics of Lampung Province. Lampung Province in Figures 2020. Lampung; 2020.
Suretno ND, Adriyani FY. The Agroindustrial Waste of Mas Kirana Banana (Musa acuminata) Packaging as Goat Feed in Lampung. In: SProceedings of the 8th National Seminar on Suboptimal Land. Palembang: Sriwijaya University Press; 2020. 978-979 p.
Ferreira IR, Junior V de AS, Almeida ÉCF, Junior FFB, Dos Santos AC, Trichez VDK, et al. Effects of Banana (Musa spp.) Bract Flour on Rats Fed High-Calorie Diet. Food Technol Biotechnol. 2023;61(2):238-249.
Umamaheswari A, Puratchikody A, Prabu SL, Jayapriya T. Phytochemical Screening and Antimicrobial Effects of Musa acuminata Bract. Int Res J Pharm. 2017; 8(8):41–44.
Al-Masri AA, Ameen F. Anti-inflammatory effect of anthocyanin-rich extract from banana bract on lipopolysaccharide-stimulated RAW 264.7 macrophages. J Funct Foods. 2023; 107:1-9.
Jiang H, Zhang W, Cao J, Jiang W. Development of biodegradable active films based on longan seed starch incorporated with banana flower bract anthocyanin extracts and applications in food freshness indication. Int J Biol Macromol. 2023; 251:1-16.
Rosa RH, Ribeiro Fernandes M, De Pádua Melo ES, Granja Arakaki D, De Lima NV, Santos Leite LC, Espindola PR, De Souza ID, Do Nascimento VA, Saldanha Tschinkel PF, La Flor Ziegler Sanches F, Do Nascimento VA. Determination of Macro- And Microelements in the Inflorescences of Banana Tree Using ICP OES: Evaluation of the Daily Recommendations of Intake for Humans. Sci World J. 2020; 2020:1-9.
Panyayong C, Srikaeo K. Foods from banana inflorescences and their antioxidant properties: An exploratory case in Thailand. Int J Gastron Food Sci. 2022; 28:1-6.
Sheng ZW, Ma WH, Gao JH, Bi Y, Zhang WM, Dou HT, Jin ZQ. Antioxidant properties of banana flower of two cultivars in china using 2,2-diphenyl-1-picrylhydrazyl (DPPH) reducing power, 2,2’-azinobis-(3-ethylbenzthiazoline-6- sulphonate (ABTS) and inhibition of lipid peroxidation assays. African J Biotechnol. 2011; 10(21):4470-4477.
Mathew NS, Negi PS. Traditional uses, phytochemistry and pharmacology of wild banana (Musa acuminata Colla): A review. J Ethnopharmacol. 2017;196(December):124-140.
Vilhena RO, Figueiredo ID, Baviera AM, Silva DB, Marson BM, Oliveira JA, Peccinini RG, Borges IK, Pontarolo, R. Antidiabetic activity of Musa x paradisiaca extracts in streptozotocin-induced diabetic rats and chemical characterization by HPLC-DAD-MS. J Ethnopharmacol. 2020; 254:1-6.
Lau BF, Kong KW, Leong KH, Sun J, He X, Wang Z, Mustafa MR, Ling TC, Ismail A. Banana inflorescence: Its bio-prospects as an ingredient for functional foods. Trends Food Sci Technol. 2020; 97:14-28.
Usman U, Masruhim MA, Kusumaningtiyas P, Erwin E, Bulan DE. Antioxidant and Antidiabetic from Rhizophora mucronata Derived from Sambera Beach, East Kalimantan, Indonesia. Trop J Nat Prod Res. 2023; 7:4921-4926.
Swallah MS, Sun H, Affoh R, Fu H, Yu H. Antioxidant Potential Overviews of Secondary Metabolites (Polyphenols) in Fruits. Int J Food Sci. 2020; 2020:1–8.
Panche AN, Diwan AD, Chandra SR. Flavonoids: an overview. J Nutr Sci. 2016 Dec 29;5(e47):1-15.
Disha Paul, Sampurna Chakraborty, Souvik Tewari, Jayashree Das. Effect of Flavonoids in Preventing Breast Cancer: A Review. J Adv Zool. 2023; 44(S6):1932-1939.
Gryn-Rynko A, Sperkowska B, Majewski MS. Screening and Structure–Activity Relationship for Selective and Potent Anti-Melanogenesis Agents Derived from Species of Mulberry (Genus Morus). Molecules. 2022; 27(24):1-16.
Hu L, Wang C, Guo X, Chen D, Zhou W, Chen X, Zhang Q. Flavonoid Levels and Antioxidant Capacity of Mulberry Leaves: Effects of Growth Period and Drying Methods. Front Plant Sci. 2021; 12:1-11.
Huang D. Dietary antioxidants and health promotion. Antioxidants. 2018; 7(1):7–9.
You SH, Jang M, Kim GH. Mori Cortex radicis extract protected against diet-induced neuronal damage by suppressing the AGE-RAGE/MAPK signaling pathway in C. elegans and mouse model. J Funct Foods. 2022; 91:1-11.
Memete AR, Timar AV, Vuscan AN, Miere F, Venter AC, Vicas SI. Phytochemical Composition of Different Botanical Parts of Morus Species, Health Benefits and Application in Food Industry. Plants. 2022; 11(2):1-29.
Hu S, Zheng Z, Chen F, Wang M. The depigmenting effect of natural resorcinol type polyphenols Kuwanon O and Sanggenon T from the roots of morus australis. J Ethnopharmacol. 2017;195(September 2015):196–203.
Jin J, Chen B, Zhan X, Zhou Z, Liu H, Dong Y. Network pharmacology and molecular docking study on the mechanism of colorectal cancer treatment using Xiao-ChaiHu-Tang. PLoS One. 2021; 16(6):1-18.
Ibrahim A, Siswandono S, Bambang PEW. Anticancer activity of Peronema canescens Jack leaves extracts against human cells: HT-29 and HeLa in vitro. Res J Pharm Technol. 2022; 15(10):4739–45.
A’yun Q, Susilo RJK, Hayaza S, Fikriyah N, Musthoza FS, Islamatasya U, et al. The Effect of G. applanatum Crude Polysaccharide Extract on Proinflammatory Cytokines and Proapoptotic Caspases in HeLa Cell Line: An In Vitro Study. Ooi DJ, editor. Adv Pharmacol Pharm Sci. 2023; 2023:1-9.
Yao X, Ph D, Lu B, Sc B, Lü C, Ph D, et al. Taraxerol Induces Cell Apoptosis through A Mitochondria-Mediated Pathway in HeLa Cells. Cell J. 2017; 19(3):512-519.
Pitakpawasutthi Y, Suwatronnakorn M, Issaravanich S, Palanuvej C, Ruangrungsi N. In vitro cytotoxic, genotoxic, and antityrosinase activities of Clitoria macrophylla root. J Adv Pharm Technol Res. 2021; 12(1):8-13.
Xiong Y, Wu X, Rao L. Tetrastigma hemsleyanum (Sanyeqing) root tuber extracts induces apoptosis in human cervical carcinoma HeLa cells. J Ethnopharmacol. 2015; 165 46-53.