Evaluation of the Antioxidant, Antinociceptive and Anti-inflammatory Activities of Combretum nigricans Ethanol Leaf Extract

Authors

  • Chinedu Enegide Department of Pharmacology & Toxicology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria
  • Peter A. Akah Department of Pharmacology & Toxicology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria
  • Adaobi C. Ezike Department of Pharmacology & Toxicology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria
  • Solomon F. Ameh Department of Pharmacology & Toxicology, National Institute for Pharmaceutical Research & Development, Idu, Abuja, Nigeria
  • Ifeoma C. Ezenyi Department of Pharmacology & Toxicology, National Institute for Pharmaceutical Research & Development, Idu, Abuja, Nigeria
  • Samuel E. Okhale Departmentof Medicinal Plant Research & Traditional Medicine, National Institute for Pharmaceutical Research & Development (NIPRD), Idu, Abuja, Nigeria

DOI:

https://doi.org/10.26538/tjnpr/v8i6.35

Keywords:

anti-inflammatory activity, antinociceptive activity, antioxidant activity, HPLC, Combretum nigricans

Abstract

Combretum nigricans is a known medicinal plant employed in Nigerian ethnomedicine for the treatment of pain, inflammation and other ailments. This study aims at evaluating the antioxidant, antinociceptive and anti-inflammatory effects of C. nigricans ethanolic leaf extract.

The antioxidant activity of C. nigricans ethanolic leaf extract was evaluated in vitro using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) model, while acetic acid writhing test and formalin nociception test were used in evaluating the antinociceptive activity in mice. Formalin-induced inflammation was used to evaluate the anti-inflammatory effect in mice. HPLC analysis of the extract showed the presence of several compounds including ferulic acid. The extract exhibited good DPPH antioxidant activity. Its IC50 value was 0.0003971 mg/mL. Oral acute toxicity test showed the LD50 is greater than 2000 mg/kg. In the acetic acid test, all extract test doses (200 – 800 mg/kg) had significant inhibitory effect against abdominal writhes usually evoked by acetic acid. The different extract doses had antinociceptive effect against phases 1 and 2 pain. Also, the extract doses used for the study (200 – 800 mg/kg) had anti-allodynia effect with the 800 mg/kg dose having a better effect of 44.44 and 79.83 % on days 1 and 2 respectively. Furthermore, the extract demonstrated significant anti-inflammatory activity against formalin induced inflammation in the experimental animals. The study outcome shows that C. nigricans extract has good antioxidant, anti-nociceptive and anti-inflammatory effects.

Author Biography

Chinedu Enegide, Department of Pharmacology & Toxicology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria

Department of Pharmacology, College of Medical & Health Sciences, Novena University, Ogume, Delta State, Nigeria

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Published

2024-06-29

How to Cite

Enegide, C., Akah, P. A., Ezike, A. C., Ameh, S. F., Ezenyi, I. C., & Okhale, S. E. (2024). Evaluation of the Antioxidant, Antinociceptive and Anti-inflammatory Activities of Combretum nigricans Ethanol Leaf Extract. Tropical Journal of Natural Product Research (TJNPR), 8(6), 7554–7560. https://doi.org/10.26538/tjnpr/v8i6.35