Antibacterial Properties of Leaves and Peels Extracts of <i>Citrus aurantifolia</i> cultivated in Algeria against Multi-Drug Resistant <i>Staphylococcus aureus</i> Originating from Raw Milk: http://www.doi.org/10.26538/tjnpr/v7i10.20

Lamia Benredjem

Authors

Lamia Benredjem Department of Molecular and Cellular Biology, Faculty of Natural and Life Sciences, Abbes Laghrour University of Khenchela, BP 1252 Road of Batna Khenchela -40004- Algeria

Keywords:

raw milk, flavonoids, polyphenols, Staphylococcus aureus, antibacterial activity, Citrus aurantifolia

Abstract

Plants have an increased consumer preference and acceptability for the treatment of several diseases. Here, the antibacterial properties of organic extracts obtained from leaves and peels of Citrus aurantifolia from Algeria have been characterized. Two solvents, methanol, and ethanol, were employed to extract the bioactive components. Quantitative analysis of total phenols and flavonoids was conducted for the different extracts. The antibacterial activity was tested against multidrug-resistant Staphylococcus aureus strains originating from raw milk and reference strains including Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, and Bacillus cereus ATCC 11778. The results revealed that leaves and peels extracts displayed significant antibacterial activity against the tested bacteria. The inhibition zone diameters observed ranged from 16.00 mm to 22.00 mm. The highest antimicrobial effect was observed with the ethanol extracts against the multidrug-resistant S. aureus strains, as indicated by a minimum inhibitory concentration of 1.56 mg/ml. The highest total phenolics and flavonoids contents were found to be 96 mg GAE/g and 54 mg QE/g in peels. Hence, the reported results unveil valuable insights into the antibacterial effects of Citrus aurantifolia extracts which have potential antimicrobial applications.

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