Exploration of Endophytic Bacteria in FIGS (Ficus carica L.) with Antibacterial Agent Potential: http://www.doi.org/10.26538/tjnpr/v7i7.10

Endang S. Gultom; Hasruddin Hasruddin; Nila Z. Wasni

Authors

Endang S. Gultom Departement of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Medan, Medan, Indonesia
Hasruddin Hasruddin Departement of Biology Education, Faculty of Mathematics and Natural Science, Universitas Negeri Medan, Medan, Indonesia.
Nila Z. Wasni Departement of Biology Education, Faculty of Mathematics and Natural Science, Universitas Negeri Medan, Medan, Indonesia.

Keywords:

16S rRNA Gene, Molecular identification, Antibacterial, Endophytic bacteria

Abstract

Tin or Ara plants are one of the plants that can be used as a source of endophytic bacteria. Figs contain phenols, benzaldehyde, terpenoids, flavonoids, alkaloids, fiber, and polyphenols. This fruit is used by the society to treat asthma, bronchitis, dry cough, diabetes, cancer, coronary heart disease, gout, and osteoporosis. In this research, endophytic bacteria will be used as a source of
bioactive compounds that have the potential to be antibacterial. The isolation of endophytic bacteria was carried out using the scatter plate method. The isolated endophytic bacteria were identified macroscopically, microscopically, and molecularly. There are 3 potential isolates that produce antibacterial compounds: TH9, TH10, and TH11. The results of bacterial species barcoding of full length of 16S rRNA bidirectional PCR sequencing of the three isolates were adjusted to the data available at the NCBI Genbank through the BLAST program. TH9 isolate had 99.86% homology with Bacillus velezensis strain NM374, TH10 isolate had 99.93% homology with Stutzerimonas stutzeri CCUG 11256 strain and TH11 isolate had 99.93% homology with Staphylococcus wareri strain CG10.

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