Caspase Dependent Inhibitory Effect of the Growth of HeLa Cells by Desmodium velutinum (Willd.) DC. Ethanol Leaf Extract
doi.org/10.26538/tjnpr/v6i1.27
Keywords:
Desmodium velutinum, Caspase, Necrosis, Cell cycle arrestAbstract
Cervical cancer is the most common cancer in women in sub-Saharan Africa and the fourth leading cause of cancer deaths in women. Adverse side effects associated with orthodox therapies have given rise to increase in natural therapies. Natural products from organisms such as plants and microbes are a rich source of therapeutic lead compounds. Desmodium velutinum (Willd.) DC. (Papilionaceae) is a perennial shrub which has been reported to be used in folkloric medicine for the treatment and management of tumours and abscesses. The anti-proliferative activity of the ethanol leaf extract of D. velutinum was assessed using MTT assay on HeLa cells. The mechanism of anti-proliferative activity was studied using cell cycle analysis, Annexin V-FITC/PI, mitochondrial membrane depolarisation, caspase 3 and 8 activation assays. velutinum ethanol leaf extract had an IC50 value of 53.27µg/mL on HeLa cells and caused accumulation of cells in the G0/G1 and early M phase. The extract also induced necrosis and led to the depolarisation of the mitochondrial membrane and activation of both caspase 3 and 8. The extract exhibited its anti-proliferative potential by mediating cell cycle arrest at the G1-S phase and cell death due to necrosis while activating the caspase system.
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