Enhancing Metabolic Parameters: The Impact of Porang Glucomannan on Body Weight, Intraperitoneal Fat, Fasting Blood Glucose, and GLUT-4 Levels in Rats Fed a High-Fat and High-Carbohydrate Diet: http://www.doi.org/10.26538/tjnpr/v7i6.20

Azizah H. Safitri; Eni Widayati; Nurina Tyagita

Authors

Azizah H. Safitri Department of Biochemistry, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang 50112, Indonesia
Eni Widayati Department of Chemistry, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang 50112, Indonesia.
Nurina Tyagita Department of Biochemistry, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang 50112, Indonesia

Keywords:

Intraperitoneal Fat, Fasting Blood Glucose, GLUT-4, Porang, Glucomannan

Abstract

Porang glucomannan (GMP) can be fermented to produce various short-chain fatty acids, which are expected to enhance insulin sensitivity and improve GLUT-4 expression. This research aims to validate the effect of porang glucomannan supplementation on body weight, intraperitoneal fat, fasting blood glucose and GLUT-4 levels in rats fed with high-fat and high-carbohydrate (HFHC) diet. The research was carried out using a posttest-only control group design, with data collected from a sample size of 30 Sprague-Dawley rats divided into five groups, each consisting of five rats, namely normal, HFHC, GMP25, GMP50, and GMP100. Except for the normal group, the other four groups were fed HFHC diet for 21 days, followed by specific treatments for each group for 28 days. The parameters measured included body weight, intraperitoneal fat weight, fasting blood glucose and GLUT-4 levels. GMP100 group demonstrated the most favourable results in fasting blood glucose levels, GLUT-4 levels, and intraperitoneal fat weight. In contrast, GMP25 group exhibited the best outcome in terms of body weight. Porang glucomannan supplementation improved body weight, blood glucose levels, GLUT-4 levels, and intraperitoneal fat in rats on HFHC diet, with the most effective dose observed at 100 mg/200 gBW.

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