In Silico and In Vitro Study of The Ethanol Extract of The White Garland Lily (Hedychium coronarium J. Koenig) as a Tyrosinase Inhibitor
http://www.doi.org/10.26538/tjnpr/v7i6.9
Keywords:
Tyrosinase, Hedychium coronarium J. Koenig, In vitro, In silicoAbstract
The Zingiberaceae family includes the White Garland Lily (Hedychium coronarium J. Koenig). It is used in traditional medicine for fever, analgesics, and indigestion. In the current study, in silico prediction of the active compound from Hedychium coronarium, analysis of the interaction with a tyrosinase inhibitor, and testing of the tyrosinase inhibitor using dopachrome compounds were done. The software used for docking simulation is Autodock-GPU. Kojic acid solution containing 1000, 500, 250, 125, and 62.5 ppm was measured into a 96-well microtiter dish to which tyrosinase enzyme solution was added, and the pH was adjusted with phosphate buffer. The mixture was incubated at 37ºC for 30 minutes before the absorbance was measured at a wavelength of 492 nm using a microplate reader. Triparanol molecules have the best bonding affinity in the molecular docking simulation data, with a -8.00 kcal/mol value. The extract yield was 3.88%, total ash content of 12.60% ± 0.27%, acid-insoluble ash content of 0.23% ± 0.02%, and water content of 5.96%. Hedychium coronarium has the potential to be developed as a Tyrosinase inhibitor from the result of the insilico investigation. Based on the result, the ethanol extract inhibited the tyrosinase enzyme with an IC50 value of 183.85 ppm compared to kojic acid with an IC50 value of 17.09 ppm.
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