Bioactive Potential of Cephalosporium sp. a Fungal Endophyte Isolated from Phyllanthus niruri L.
http://www.doi.org/10.26538/tjnpr/v7i4.13
Keywords:
Breast cancer, Cephalosporium sp., Phyllanthus niruri, EndophyteAbstract
Fungal endophytes are fungi that reside within plant tissues without causing apparent harm to their hosts. These fungi produce various bioactive compounds that have potential applications in medicine. This study aimed to cultivate a Cephalosporium sp. fungal endophyte isolated from Phyllanthus niruri L., and evaluate its antimicrobial, antioxidant, and cytotoxic activities in the endophytic fungal extract. The endophyte was extracted, fractionated, and assessed for total phenolic content, antimicrobial, and antioxidant activities, as well as potential as an anti-breast cancer agent against 4T1 cell lines. Results showed that the dichloromethane fraction effectively inhibited the growth of Salmonella typhi with an IC50 value of 12.39 µg/mL. In contrast, the methanol fraction exhibited high antioxidant activity with a free radical scavenging ability of 86%. Moreover, the dichloromethane fraction demonstrated the ability to inhibit 4T1 cell growth with an IC50 value of 18.12 µg/ml. These findings suggest that the dichloromethane fraction has the potential as an antimicrobial and cytotoxic agent against breast cancer cells.
References
Yuan Z, Tian Y, He F, Zhou H. Endophytes from Ginkgo biloba and their secondary metabolites. Chin Med. 2019;14:51.
Astuti P, Rollando R, Wahyuono S, Nurrochmad A. Antimicrobial activities of isoprene compounds produced by an endophytic fungus isolated from the leaves of Coleus amboinicus Lour. J Pharm Pharmacogn Res. 2020;8(4):280–9.
Ancheeva E, Daletos G, Proksch P. Bioactive Secondary Metabolites from Endophytic Fungi. Curr Med Chem. 2020;27(11):1836–54.
Klykleung N, Yuki M, Kudo T, Ohkuma M, Phongsopitanun W, Pittayakhajonwut P, Tanasupawat S. Nonomuraea phyllanthi sp. nov., an endophytic actinomycete isolated from the leaf of Phyllanthus amarus. Arch Microbiol. 2020;202(1):55–61.
Niemhom N, Chutrakul C, Suriyachadkun C, Thawai C. Nonomuraea stahlianthi sp. nov., an endophytic actinomycete isolated from the stem of Stahlianthus campanulatus. Int J Syst Evol Microbiol. 2017;67(8):2879–84.
Lee NYS, Khoo WKS, Adnan MA, Mahalingam TP, Fernandez AR, Jeevaratnam K. The pharmacological potential of Phyllanthus niruri. J Pharm Pharmacol. 2016;68(8):953–69.
Dhawan S, Olweny EO. Phyllanthus niruri (stone breaker) herbal therapy for kidney stones; a systematic review and meta-analysis of clinical efficacy, and Google Trends analysis of public interest. Can J Urol. 2020;27(2):10162–6.
Iskandar D, Widodo N, Warsito W, Masruri M, Rollando R, Warsidah W, Antang YPP. Proposed Functional Activity of Bioactive Compounds from Spatholobus littoralis Hassk in LC-MS-MS and in Silico Studies. Mater Sci Forum. 2022;1061:181–6.
Hariono M, Rollando R, Karamoy J, Hariyono P, Atmono M, Djohan M, Wiwy W, Nuwarda R, Kurniawan C, Salin N, Wahab H. Bioguided Fractionation of Local Plants against Matrix Metalloproteinase9 and Its Cytotoxicity against Breast Cancer Cell Models: In Silico and In Vitro Study. Mol Basel Switz. 2020;25(20):E4691.
Hariono M, Nuwarda RF, Yusuf M, Rollando R, Jenie RI, Al-Najjar B, Julianus J, Putra KC, Nugroho ES, Wisnumurti YK, Dewa SP, Jati BW, Tiara R, Ramadani RD, Qodria L, Wahab HA. Arylamide as Potential Selective Inhibitor for Matrix Metalloproteinase 9 (MMP9): Design, Synthesis,
Biological Evaluation, and Molecular Modeling. J Chem Inf Model. 2020;60(1):349–59.
Rollando R. Combination of Hedyotis corymbosa l. and Tinospora crispa Ethanolic Extract Increase Cisplatin Cytotoxicity on T47D Breast Cancer Cells. Asian J Pharm Clin Res. 2018;171–7.
Coleman AW. Nuclear rRNA transcript processing versus internal transcribed spacer secondary structure. Trends Genet TIG. 2015;31(3):157–63.
Wang X, Tian Z, Xi Y, Guo Y. Identification of endophytic fungi with ACC deaminase-producing isolated from halophyte Kosteletzkya Virginica. Plant Signal Behav. 2022;17(1):2152224.
Qadri M, Johri S, Shah BA, Khajuria A, Sidiq T, Lattoo SK, Abdin MZ, Riyaz-Ul-Hassan S. Identification and bioactive potential of endophytic fungi isolated from selected plants of the Western Himalayas. SpringerPlus. 2013;2(1):8-14.
Thi Minh Le T, Thi Hong Hoang A, Thi Bich Le T, Thi Bich Vo T, Van Quyen D, Hoang Chu H. Isolation of endophytic fungi and screening of Huperzine A-producing fungus from Huperzia serrata in Vietnam. Sci Rep. 2019;9(1):16152.
Soni SK, Singh R, Ngpoore NK, Niranjan A, Singh P, Mishra A, Tiwari S. Isolation and characterization of endophytic fungi having plant growth promotion traits that biosynthesizes bacosides and withanolides under in vitro conditions. Braz J Microbiol Publ Braz Soc Microbiol. 2021;52(4):1791–805.
Shadmani L, Jamali S, Fatemi A. Isolation, identification, and characterization of cadmium-tolerant endophytic fungi isolated from barley (Hordeum vulgare L.) roots and their role in enhancing phytoremediation. Braz J Microbiol Publ Braz Soc Microbiol. 2021;52(3):1097–106.
Rollando R, Sitepu R, Monica E. Effects of solvent composition on Profile of total extracts, phenolic compounds, and antifungal activity of Sterculia quadrifida R.Br Bark. J Glob Pharma Technol. 2019;11(Issue 01 (Suppl.)):342–51.
Yuniati Y, Yuliati L, Monica E, Rollando R. Discovering anticancer compound of ethyl acetate extract from RL1 code endophytic fungi culture derived by Phyllanthus niruri Linn leaves through cell cycle modulation in T47d cells. IOP Conf Ser Mater Sci Eng. 2019;509:012157.
Rollando R, Warsito W, Masruri M, Nashi W. Antibacterial, Antioxidant, and Cytotoxic Flavonoid Compound from Sterculia Quadrifida Leaves. Trop J Nat Prod Res. 2021;5(11):1979–85.
Kuo J, Chang CF, Chi WC. Isolation of endophytic fungi with antimicrobial activity from medicinal plant Zanthoxylum simulans Hance. Folia Microbiol (Praha). 2021;66(3):385–97.
Rollando R, Sitepu R, Eva M. Cytotoxic activity of 2-iminoethyl 2-(2-(1-hydroxypentan-2-yl) phenyl) acetate from Sterculia quadrifida R. Br ethyl acetate fraction. J Glob Pharma Technol. 2018;10(6):204–12.
Rollando R, Warsito W, Masruri M, Widodo W. Pterygota alata (Roxb.) R.Br. Bark Fraction Induced Intrinsic Apoptotic Pathway in 4T1 Cells by Decreasing Bcl-2 and Inducing Bax Expression. Pak J Biol Sci PJBS. 2021;24(2):172–81.
He J, Xu L, Yang L, Wang X. Epigallocatechin Gallate Is the Most Effective Catechin Against Antioxidant Stress via Hydrogen Peroxide and Radical Scavenging Activity. Med Sci Monit Int Med J Exp Clin Res. 2018;24:8198–206.
Kut K, Bartosz G, Soszyński M, Sadowska-Bartosz I. Antioxidant properties of hispidulin. Nat Prod Res. 2022;36(24):6401–4.
Hariono M, Rollando R, Yoga I, Harjono A, Suryodanindro A, Yanuar M, Gonzaga T, Parabang Z, Hariyono P, Febriansah R, Hermawansyah A, Setyani W, Wahab H. Bioguided Fractionation of Local Plants against Matrix Metalloproteinase9 and Its Cytotoxicity against Breast Cancer Cell Models: In Silico and In Vitro Study (Part II). Mol Basel Switz. 2021;26(5):1464.
Rollando R, Warsito W, Masruri M, Widodo N. Potential matrix metalloproteinase-9 inhibitor of aurone compound isolated from Sterculia quadrifida leaves: In-vitro and insilico studies. Res J Pharm Technol. 2022;15(11):5250–4.
Rollando R, Monica E, Aftoni MH. In vitro Cytotoxic Potential of Sterculia quadrifida Leaf Extract Against Human Breast Cancer Cell Lines. Trop J Nat Prod Res. 2022;6(8):1228–32.
Han L, Zhou X, Zhao Y, Zhu S, Wu L, He Y, Ping X, Lu X, Huang W, Qian J, Zhang L, Jiang X, Zhu D, Luo C, Li S, Dong Q, Fu Q, Deng K, Wang X, Wang L, Peng S, Wu J, Li W, Friml J, Zhu Y, He X, Du Y. Colonization of endophyte Acremonium sp. D212 in Panax notoginseng and rice
mediated by auxin and jasmonic acid. J Integr Plant Biol. 2020;62(9):1433–51.
Lyu JI, Ryu J, Jin CH, Kim DG, Kim JM, Seo KS. Phenolic Compounds in Extracts of Hibiscus acetosella (Cranberry Hibiscus) and Their Antioxidant and Antibacterial Properties. Mol Basel Switz. 2020;25(18):4190.
Erharuyi O, Engel N, Ahomafor J, Imieje V, Falodun A, Babara N, Langer P. Anticancer Activity of Five Forest Crops Used in African Folklore: Antiproliferative and ProApoptotic Effects. Nat Prod Res. 2014;1-6.
Lutz NE, Falodun A, Kuhn J, Kragl U, Langer P, Nebe B. Pro-apoptotic and Anti-Adhesive Effect of Four African Plant Extracts on The Breast Cancer Cell Line MCF-7. BMC Complem Altern Med. 2014;334(14):1-13. Engel N, Oppermann C, Falodun A, Kragl U.
Proliferative Effects of Five Traditional Nigerian Medicinal Plant Extracts on Human Breast and Bone Cancer Cell Lines. J Ethnopharmacol. 2011;137:1003-1010.
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Tropical Journal of Natural Product Research (TJNPR)
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
