Bioactive Potential of Cephalosporium sp. a Fungal Endophyte Isolated from Phyllanthus niruri L.


  • Rollando Rollando Program of Pharmacy, Faculty of Science and Technology, Ma Chung University, Malang 65151, Indonesia
  • Yuyun Yuniati Program of Chemistry, Faculty of Science and Technology, Ma Chung University, Malang 65151, Indonesia
  • Eva Monica Program of Pharmacy, Faculty of Science and Technology, Ma Chung University, Malang 65151, Indonesia


Breast cancer, Cephalosporium sp., Phyllanthus niruri, Endophyte


Fungal endophytes are fungi that reside within plant tissues without causing apparent harm to their hosts. These fungi produce various bioactive compounds that have potential applications in medicine. This study aimed to cultivate a Cephalosporium sp. fungal endophyte isolated from Phyllanthus niruri L., and evaluate its antimicrobial, antioxidant, and cytotoxic activities in the endophytic fungal extract. The endophyte was extracted, fractionated, and assessed for total phenolic content, antimicrobial, and antioxidant activities, as well as potential as an anti-breast cancer agent against 4T1 cell lines. Results showed that the dichloromethane fraction effectively inhibited the growth of Salmonella typhi with an IC50 value of 12.39 µg/mL. In contrast, the methanol fraction exhibited high antioxidant activity with a free radical scavenging ability of 86%. Moreover, the dichloromethane fraction demonstrated the ability to inhibit 4T1 cell growth with an IC50 value of 18.12 µg/ml. These findings suggest that the dichloromethane fraction has the potential as an antimicrobial and cytotoxic agent against breast cancer cells. 


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How to Cite

Rollando, R., Yuniati, Y., & Monica, E. (2023). Bioactive Potential of Cephalosporium sp. a Fungal Endophyte Isolated from Phyllanthus niruri L.: Tropical Journal of Natural Product Research (TJNPR), 7(4), 2749–2755. Retrieved from

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