Efficacy of Nitraria retusa L. Fruits Aqueous and Methanol Extracts as Antioxidant and Anti-inflammatory Activities on Carrageenan-Induced Paw Edema in Rats.
http://www.doi.org/10.26538/tjnpr/v7i4.9
Keywords:
Inflammation, Paw edema, Diphenyl-2-picryl-hydrazyl, Nitraria retusa, aAbstract
The aim of this study was to assess the anti-oxidant and anti-inflammatory activities of aqueous and methanol extracts of Nitraria retusa against carrageenan induced paw edema in rats. The extracts were studied for antioxidant activity using Diphenyl-2-picryl-hydrazyl assay. Male Wister rats were divided into six groups including positive and negative controls. Group 1 (normal saline) as negative control, group 2 (150 mg/kg acetylsalicylic acid) as positive control, group 3 (100 mg/kg methanolic extract), group 4 (200 mg/kg methanolic extract), group 5 (100 mg/kg aqueous extract), group 6 (200 mg/kg aqueous extract). The results showed that aqueous and methanolic extract at doses of 100 and 200 mg/kg significantly decrease paw edema (P˂0.05). Also, the results revealed that methanol extract at 200 mg/kg is more effective in inhibiting paw edema (P˂0.005) than other doses and is equal to efficacy of acetylsalicylic acid. This finding indicated that N. retusa has an anti-inflammatory activity against carrageenan induced paw edema in rats. DPPH assay showed that methanol extract of N. retusa has high antioxidant activity (958.2±178.3 µg/mL) compared to aqueous extract (1249.3±233.5 µg/mL). In conclusion, N. retusa fruit extracts have an antioxidant activity and anti-inflammatory activity against carrageenan induced paw edema.
References
Sun Q, Zhu J, Cao F, Chen F. Anti-inflammatory properties
of extracts from Chimonanthus nitens Oliv. leaf. PLoS
ONE. 2017; 12(7):1-19.
Furman D, Campisi J, Verdin E, Carrera-Bastos P, Targ S,
Franceschi C, Ferrucci L, Gilroy DW, Fasano A, Miller
GW, Miller AH, Mantovani A, Weyand CM, Barzilai N,
Goronzy JJ, Rando TA, Effros RB, Lucia A, Kleinstreuer N,
Slavich GM. Chronic Inflammation in the Etiology of
Disease Across the Life Span. Nat. Med. 2019;
(12):1822–1832.
Bindu S., Mazumder S., and Bandyopadhyay U. Nonsteroidal anti-inflammatory drugs (NSAIDs) and organ
damage: A current perspective. Biochem. Pharmacol. 2020;
:114-147.
Aminah NS, Tun KNW, Kristanti AN, Aung HT, Takaya Y,
Choudhary MI. Chemical constituents and their biological
activities from Taunggyi (Shan state) medicinal plants.
Heliyon. 2021; 7(2):e06173.
De La Cruz-Jiménez L, Hernández-Torres MA, MonroyGarcía IN, Rivas-Morales C, Verde-Star MJ, GonzalezVillasana V, Viveros-Valdez E. Biological Activities of
Seven Medicinal Plants Used in Chiapas, Mexico. Plants
(Basel). 2022; 11(14):1790-1802.
Oran S and Zeidan R. Antioxidant activity of leaf and fruit
extracts of Jordanian Rubus sanguineus Friv. (Rosaceae).
J. Med. Plant Res. 2014; 8(39): 1179–1190.
7Al-Eisawi D M H. Flora of Jordan Checklist, Revised;
Jafri S., El-Gadi A. Flora of Libya. Botany Department,
Faculty of Science, Al Fatteh University, Tripoli; 1977.
Boubaker J, ben Toumia I, Sassi A, Bzouich-Mokded I.,
Ghoul Mazgar S, Sioud F, Bedoui A, Safta Skhiri S,
Ghedira K, & Chekir-Ghedira L. Antitumoral Potency by
Immunomodulation of Chloroform Extract from Leaves of
Nitraria retusa, Tunisian Medicinal Plant, via its Major
Compounds β-sitosterol and Palmitic Acid in BALB/c Mice
Bearing Induced Tumor. Nutr Cancer. 2018; 70(4):650–
Chaâbane M, Koubaa M, Soudani N, Elwej A, Grati M,
Jamoussi K, Boudawara T, Chaabouni S E, Zeghal N.
Nitraria retusa fruit prevents penconazole-induced kidney
injury in adult rats through modulation of oxidative stress
and histopathological changes. Pharm. Biol. 2017; 55(1):
–1073.
Chaâbane M, Soudani N, Benjeddou K, Turki M, Ayadi
Makni F, Boudawara T, Zeghal N, & Ellouze Ghorbel R.
The protective potential of Nitraria retusa on penconazoleinduced hepatic injury in adult rats. Toxicol Environ Chem.
; 97(9): 1253–1264.
Hussein SR, Kawashty SA, Tantawy
ME. Chemosystematic studies of Nitraria retusa and
selected taxa of Zygophyllaceae in Egypt. Plant Syst Evol.
; 277:251–264.
Majoumouo MS, Marius BT, Thureyah M, Donavon CH,
Fabrice FB, Chitra M. Antiproliferative potential of
methanolic and aqueous extracts and their methanolic
fractions derived from fruits of Bersama engleriana against
a panel of four cancer cell lines, Cogent Biol. 2020; 6(1):1-
Siddiqui N, Rauf A, Latif A, Mahmood Z.
Spectrophotometric determination of the total phenolic
content, spectral and fluorescence study of the herbal Unani
drug Gul-e-Zoofa (Nepeta bracteata Benth). J Taibah Univ
Med Sc. 2017; 12(4):360-363.
Chang CC, Yang H, Wen HM, Chern JC. Estimation of
Total Flavonoid Content in Propolis by Two
Complementary Colorimetric Methods. J Food Drug Anal.
; 10(3):178-182.
Paulpriya K, Lincy MP, Tresina PS, Mohan VR. In Vitro
Antioxidant Activity, Total Phenolic and Total Flavonoid
Contents of Aerial Part Extracts of Daphniphyllum
Neilgherrense (Wt.) Rosenth. J Biol Innov. 2015; 4(6):257-
Bolla SR, Mohammed Al-Subaie A, Yousuf Al-Jindan R,
Papayya Balakrishna J, Kanchi Ravi P, Veeraraghavan VP,
Arumugam Pillai A, Gollapalli SSR, Palpath Joseph J,
Surapaneni KM. In vitro wound healing potency of
methanolic leaf extract of Aristolochia saccata is possibly
mediated by its stimulatory effect on collagen-1 expression.
Heliyon. 2019; 5(5):e01648.
Amole OO, Akinyede AA, Obanyero DD. Toxicity Studies
of the Hydroethanolic Leaf Extract of Clerodendrum
polycephalum (Lamiaceae) in Rats. Ann. clin. Toxicol.
; 4(1):1-6.
Rajput MA, Zehra T, Ali F, Kumar G. Evaluation of
antiinflammatory activity of ethanol extract of Nelumbo
nucifera fruit. Turk J Pharm Sci. 2021; 18(1):56–60.
Hisamuddin N, Shaik Mossadeq WM, Sulaiman MR, Abas
F, Leong SW, Kamarudin N, Ong HM, Ahmad Azmi AF,
Ayumi RR, Talib M. Anti-Edematogenic and AntiGranuloma Activity of a Synthetic Curcuminoid Analog, 5-
(3,4-Dihydroxyphenyl)-3-hydroxy-1-(2-
hydroxyphenyl)penta-2,4-dien-1-one, in Mouse Models of
Inflammation. Molecules. 2019; 24(14):2614-2628.
Hossain MA, Shah MD, Gnanaraj C, Iqbal M. In vitro total
phenolics, flavonoids contents and antioxidant activity of
essential oil, various organic extracts from the leaves of
tropical medicinal plant Tetrastigma from Sabah. Asian Pac
J Trop Med. 2011; 4(9):717-721.
Abdulkhaleq LA, Assi MA, Abdullah R, Zamri-Saad M,
TaufiqYap YH, Hezmee M. The crucial roles of inflammatory mediator in inflammation: A review. Vet
World. 2018; 11(5):627-635.
Sadeghi H, Hajhashemi V, Minaiyan M, Movahedian A,
Talebi A. Further studies on anti-inflammatory activity of
maprotiline in carrageenan-induced paw edema in rat. Int.
Immunopharmacol.. 2013; 15(3):505-510.
Rjeibi I, Hentati F, Feriani A, Hfaiedh N, Delattre C,
Michaud P, Pierre G. Novel Antioxidant, Anti-α-Amylase,
Anti-Inflammatory and Antinociceptive Water-Soluble
Polysaccharides from the Aerial Part of Nitraria retusa.
Foods. 2019; 9(1): 28-45.
Alsarayreh A, Oran S, Shakhanbeh J. Evaluation of Antiinflammatory Activity of Methanol Extract of Rhus coriaria
L. in Diabetic Rats. Trop. J. Nat. Prod. Res. 2021; 5(8):
–1413.
Al-Bzour J, Oran S, Mashallah K, Bustanji Y. Inhibitory
effects of methanolic extract of Salvia fruticosa Mill. on
pro-inflammatory cytokines production in RAW 264.7 in
vitro cellular model and in Balb/c mice in vivo animal
model. J. Med. Plant Res. 2011; 5(30):6662-6671.
Alsarayreh AZ, Oran S A, Shakhanbeh JM, Khleifat K M.,
Al Qaisi YT., Alfarrayeh I I, Alkaramseh AM. Efficacy of
methanolic extracts of some medicinal plants on wound
healing in diabetic rats. Heliyon. 2020; 8(8):e10071.
Al Qaisi YT., Khleifat KM., Alfarrayeh I I, Alsarayreh A Z.
In Vivo Therapeutic Effect of Some Medicinal Plants’
Methanolic Extracts on the Growth and Development of
Secondary Hydatid Cyst Infection. Acta Parasitol. 2020; 1-
Al Qaisi YT, Khleifat KM, Oran SA, Al Tarawneh AA,
Qaralleh H, Al-Qaisi TS, Farah HS. Ruta graveolens,
Peganum harmala, and Citrullus colocynthis methanolic
extracts have in vitro protoscolocidal effects and act against
bacteria isolated from echinococcal hydatid cyst fluid. Arch.
Microbiol. 2022; 204(4):228-231.
Falodun A, Okunrobo LO and Uzoamaka
N. Phytochemical screening and anti-inflammatory
evaluation of methanolic and aqueous extracts of Euphorbia
heterophylla Linn (Euphorbiaceae). Afri J
Biotechnol. 2006; 5, 529 - 531.
Falodun A, Okunrobo LO and Agbo LO. Evaluation of the
anti-edematogenic activity of Leea guineensis leaf. Afri J
Biotechnol 2007; 6 (9):1151–1153.
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