Antibacterial and Antioxidant Activities of Extracts from Selected Wild Plant Species Found in Jordan

http://www.doi.org/10.26538/tjnpr/v7i3.8

Authors

  • Salameh Alqaraleh Department of Nutrition and Food Technlolgy. University of Jordan, Amman 11942, Jordan
  • Ghadeer F. Mehyar Department of Nutrition and Food Technlolgy. University of Jordan, Amman 11942, Jordan
  • Moath Alqaraleh Department of Nutrition and Food Technlolgy. University of Jordan, Amman 11942, Jordan
  • Saddam S. Awaisheh Department of Nutrition and Food Science, Al-Balqa Applied University, 19117, Balqa, Jordan
  • Razan J. Rahahleh Department of Nutrition and Food Science, Al-Balqa Applied University, 19117, Balqa, Jordan

Keywords:

Urtica pilulifera L, Rumex vesicarus L, Rubus canescens DC, Methanol extracts,, Antioxidants, Antibacterial

Abstract

In the past decade, there has been a growing global interest in the antibacterial and antioxidant properties of plants, which are considered safe for use. This study aimed to evaluate the ntibacterial and antioxidant activities, as well as the flavonoid and total  henolic contents, of three wild plant species found in Jordan: Rubus canescens DC, Rumex vesicarus L, and Urtica pilulifera L. The 70%  methanol extracts of these plants were tested for their antioxidant activity using various assays, such as total phenols, free radical  scavenging, and reducing power. Results showed that Rubus canescens DC extract had a higher percentage of total phenols and free  radical scavenging activity, while Rumex vesicarus L extract had a higher percentage of reducing power. The extracts' antimicrobial  activity was evaluated using a two-fold dilution method, including Minimum Inhibitory Concentration (MIC) and Minimal Bactericidal  Concentration (MBC) activity. The extract of Rubus canescens DC showed higher antibacterial activity against the tested microorganisms, as measured by inhibition zones in plate-diffusion assays. These findings suggest that Rubus canescens DC could be  a potential source of natural antioxidants and warrant further investigation of its therapeutic properties. 

Author Biography

Moath Alqaraleh, Department of Nutrition and Food Technlolgy. University of Jordan, Amman 11942, Jordan

Department of Applied Biology, Faculty of Science, Tafila Technical University, 66110 Tafila, Jordan

 

References

Bourgaud F, Gravot A, Milesi S, Gontier E. Production of plant secondary metabolites: a historical perspective. Plant Sci. 2001; 161(5):839-851.

Dmour SM, Qaralleh H, Al-Limoun M, Khleifat KM, Alqaraleh M, Alqudah AA, Altarawneh RM. Combined Antibacterial activity of Eucalyptol, γ- terpinene, p-cymol and punicalagin with Cefotaxime against Methicillin (Oxacillin) Resistant Staphylococcus aureus Isolate. Res. J. Pharm. Technol. 2022; 15(9): 3905-3911.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, RiceEvans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol. Med. 1999; 26(9-10): 1231-1237.

Al-Tawarah NM, Qaralleh H, Khlaifat AM, Nofal MN, Alqaraleh M, Khleifat KM, Al-limoun MO, Al Shhab MA. Anticancer and Antibacterial Properties of Verthemia Iphionides Essential Oil/Silver Nanoparticles. Biomed. Pharmacol. J. 2020;13(3): 1175-1185.

Weldegerima B. Review on the importance of documenting ethnopharmacological information on medicinal plants. Afr. J. Pharm. Pharmacol. 2009; 3(9):400-403.

Alqaraleh M, Kasabri V. The antiglycation effect of monomethyl branched chained fatty acid and phytochemical compounds and their synergistic effect on obesity related colorectal cancer cell panel. Rom J Diabetes Nutr Metab Dis. 2019; 26(4): 361-369.

Alhabashneh W, Khleifat KM, Alqaraleh M, Al-Omari L, Qinna N, Al-limoun MO, Qaralleh H, Farah HS, Alqais T. Evaluation of the Therapeutic Effect of Curcumin Phytosomes on Streptozotocin-Induced Diabetic Rats. TJNPR. 2022; 6(4): 529-536.

Alqaraleh M, Kasabri V, Aljaafreh A, Al-Majali I, AlOthman N, Khleifat K, Khaleel S, Al‐Tawarah N, Qaralleh H, Al-majali M. Evaluation of the antiglysation effect of branched chain amino acids and phytochemical compounds on RAW 264.7 cell line and their synergistic effect on colorectal cell line panel. TJNPR. 2021; 5(1):88-93.

Al-Soub A, Khleifat K, Al-Tarawneh A, Al-Limoun M, Alfarrayeh I, Al Sarayreh A, Al Qaisi Y, Qaralleh H, Alqaraleh M, Albashaireh A. Silver nanoparticles biosynthesis using an airborne fungal isolate, Aspergillus flavus: optimization, characterization and antibacterial activity. Iran J Microbiol. 2022; 14(4).

Altiti AJ, Khleifat KM, Alqaraleh M, Shraim AaS, Qinna N, Al-Tawarah NM, Al-Qaisi TS, Aldmour RH, Al-Tarawneh A, Qaralleh H. Protective Role of Combined Crataegus Aronia Ethanol Extract and Phytosomes Against Hyperglycemia and Hyperlipidemia in StreptozotocinInduced Diabetic Rat. Biointerface Res. Appl. Chem. 2022.

Hajleh MNA, Khleifat KM, Alqaraleh M, Al-Hraishat E, Allimoun MO, Qaralleh H, Al-Dujaili EA. Antioxidant and Antihyperglycemic Effects of Ephedra foeminea Aqueous Extract in Streptozotocin-Induced Diabetic Rats. Nutrients. 2022; 14(11):2338.

Khwaldeh A, Shraideh Z, Badran D, Alzbeede A, Farajallah M, AlQattan D. Ameliorative Effect of Turmeric and Cocoa Extract against Acute Second Hand Exposure of Tobacco Smoking on Hepatocytes and Enterocytes in Albino Rats: Ultrastructural Study. Biomed. Pharmacol. J. 2021;14(1):199-206.

Yousef I, Oran S, Alqaraleh M, Bustanji M. Evaluation of cytotoxic, antioxidant and antibacterial activities of Origanum dayi, Salvia palaestina and Bongardia chrysogonum plants growing wild in Jordan. TJNPR. 2021;5(1):66-70.

Al-Nabulsi A, Osaili T, Olaimat A, Almasri W, Al-Holy M, Jaradat Z, Ayyash M, Awaisheh S, Holley R. Inhibitory effect of thyme and cinnamon essential oils against E. coli O157: H7 in Tahini. Food Sci. Technol. 2020; 40(4): 885- 893.

Tonthubthimthong P, Chuaprasert S, Douglas P, Luewisutthichat W. Supercritical CO2 extraction of nimbin from neem seeds–an experimental study. J. Food Eng. 2001; 47(4): 289-293.

Fratianni F, De Martino L, Melone A, De Feo V, Coppola R, Nazzaro F. Preservation of chicken breast meat treated with thyme and balm essential oils. J. Food Sci.. 2010; 75(8):M528-M535.

Agbor GA, Oben JE, Ngogang JY. Haematinic activity of Hibiscus cannabinus. Afr. J. Biotechnol. 2005; 4(8): 833- 837.

Gulcin İ. Antioxidants and antioxidant methods: An updated overview. Arch. Toxicol. 2020; 94(3):651-715.

Al-Eisawi D. Flora of Jordan Checklist, Revised. The University of Jordan Press, Amman, Jordan; 2013.

Oran SA. The status of medicinal plants in Jordan. J. Agric. Sci. Technol. A. 2014;4(6A).

Escuredo O, Silva LR, Valentão P, Seijo MC, Andrade PB. Assessing Rubus honey value: Pollen and phenolic compounds content and antibacterial capacity. Food Chem. 2012;130(3):671-678.

Fu Y, Zhou X, Chen S, Sun Y, Shen Y, Ye X. Chemical composition and antioxidant activity of Chinese wild raspberry (Rubus hirsutus Thunb.). LWT- Food Sci. Technol. 2015;60(2):1262-1268.

Bilto YY, Alabdallat NG. Ex vivo and In vivo antioxidant related effects of zingiber officinale roscoe (Ginger) extracts in humans. J Med Plants. 2015; 7(2):99.

Slinkard K, Singleton VL. Total phenol analysis: automation and comparison with manual methods. Am J Enol Vitic. 1977;28(1):49-55.

Hatano T, Kagawa H, Yasuhara T, Okuda T. Two new flavonoids and other constituents in licorice root: their relative astringency and radical scavenging effects. Chem. Pharm. Bull. 1988;36(6):2090-2097.

Awaisheh SS, Ibrahim SA. Screening of antibacterial activity of lactic acid bacteria against different pathogens found in vacuum-packaged meat products. Foodborne Pathog. Dis. 2009;6(9): 1125-1132.

Al-Nabulsi AA, Awaisheh SS, Osaili TM, Olaimat AN, Rahahaleh RJ, Al-Dabbas FM, Al-Kharabsheh LA, Gyawali R, Ibrahim SA. Inactivation of Cronobacter sakazakii in reconstituted infant milk formula by plant essential oils. J. Appl. Bot. Food Qual. 2015; 88(1). 97-101

Awaisheh SS. Efficacy of Fir and Qysoom essential oils, alone and in combination, in controlling Listeria monocytogenes in vitro and in RTE meat products model. Food control. 2013; 34(2):657-661.

Assafiri O, Abdallah H, El-Dakdouki M. Antibacterial effect and phytochemical analysis of the shoot system of Rubus canescens DC. growing in

Lebanon. BAU J. Sci. Technol. 2020;2(1):9.

Salim GM, El-Dakdouki MH, Abdallah H, Nasser HM, Arnold-Apostolides N. Antioxidative and Hepatoprotective Effects of Rubus canescens DC. Growing Wild in Lebanon. J. Nat. Prod. 2021; 11(1):44-56.

Jayanthi G, Kamalraj S, Karthikeyan K, Muthumary J. Antimicrobial and antioxidant activity of the endophytic fungus Phomopsis sp. GJJM07 isolated from Mesua ferrea. Int J Curr Sci. 2011; 1: 85-90.

Oyedeji O, Afolayan A. Chemical composition and antibacterial activity of the essential oil of Centella asiatica. Growing in South Africa. Pharm. Biol. 2005; 43(3) :249-252.

Panizzi L, Caponi C, Catalano S, Cioni P, Morelli I. In vitro antimicrobial activity of extracts and isolated constituents of Rubus ulmifolius. J. Ethnopharmacol. 2002; 79(2) :165-168.

Basile A, Sorbo S, Spadaro V, Bruno M, Maggio A, Faraone N, Rosselli S. Antimicrobial and antioxidant activities of coumarins from the roots of Ferulago campestris (Apiaceae). Molecules. 2009; 14(3):939-952.

Gyawali R, Ibrahim SA. Natural products as antimicrobial agents. Food control. 2014; 46:412-429.

Dorman HD, Deans SG. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J. Appl. Microbiol. 2000; 88(2): 308-316.

Chanda S, Deb L, Tiwari RK, Singh K, Ahmad S. Gastroprotective mechanism of Paederia foetida Linn.(Rubiaceae)–a popular edible plant used by the tribal community of North-East India. BMC Complementary Altern. Med. 2015; 15(1):1-9

Published

2023-03-31

How to Cite

Alqaraleh, S., Mehyar, G. F., Alqaraleh, M., Awaisheh, S. S., & Rahahleh, R. J. (2023). Antibacterial and Antioxidant Activities of Extracts from Selected Wild Plant Species Found in Jordan: http://www.doi.org/10.26538/tjnpr/v7i3.8. Tropical Journal of Natural Product Research (TJNPR), 7(3), 2520–2524. Retrieved from https://tjnpr.org/index.php/home/article/view/1779

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