The Potential of Lampung Robusta Green Coffee (Coffea Canephora) Extract Toward T Cell Activation in ISA-brown Laying Chickens

Authors

  • Djalal Rosyidi Animal Product Technology Laboratory, Faculty of Animal Science, Universitas Brawijaya, Malang, Indonesia
  • Lilik E. Radiati Animal Product Technology Laboratory, Faculty of Animal Science, Universitas Brawijaya, Malang, Indonesia
  • Dahliatul Qosimah Laboratory of Microbiology and Immunology, Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, Indonesia
  • Indah A. Amri Laboratory of Microbiology and Immunology, Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, Indonesia
  • Dodik Prasetyo Animal Clinical, Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, Indonesia
  • Agri K. Anisa Laboratory of Pharmacology, Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, Indonesia

Keywords:

Antioxidant, T lymphocytes, Chicken, Coffee, Immunomodulator

Abstract

Coffee (Coffea canephora) is one of the world's most popular beverages containing active chemicals such as caffeine, phenol, trigonelline, diterpenes, and water-soluble substances. Chlorogenic acids are a type of phenol found in coffee that can stimulate the immune system within the body. As a result, this natural substance promotes poultry growth by strengthening the immune system and preventing disease. There is a dearth of information on coffee as an immunomodulator in chickens, necessitating this  investigation. The present study was therefore conducted to investigate the effect of Lampung Robusta green coffee extract on T lymphocyte cell activation in ISA-brown laying chickens. Ethanolic extract of the Lampung Robusta green coffee was prepared and subjected to liquid chromatography-mass spectrometric analysis. One-day-old ISA-brown laying chickens were divided into four groups: control (C), T1, T2, and T3 groups, which received extract coffee doses of 500, 1000, and 1500 mg/kg BW, respectively. The relative numbers of TCD4+ , CD8+ , and CD45+ cells were determined using a flow cytometer. The results of the LC-MS analysis revealed that the Lampung Robusta green coffee extract also contained chlorogenic acids. With a dose of 1500 mg/kg BW, Lampung Robusta green coffee extract had an immunomodulatory effect on the T3 group, increasing the relative number of TCD4+ and CD45+ while decreasing CD8+ . The relative number of TCD8+ was higher than that of TCD4+ , suggesting immunosuppressive potential. The findings of this study revealed that Lampung Robusta green coffee extract is effective as an immunomodulator in chickens.

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Published

2022-06-01

How to Cite

Rosyidi, D., E. Radiati, L., Qosimah, D., A. Amri, I., Prasetyo, D., & K. Anisa, A. (2022). The Potential of Lampung Robusta Green Coffee (Coffea Canephora) Extract Toward T Cell Activation in ISA-brown Laying Chickens. Tropical Journal of Natural Product Research (TJNPR), 6(6), 875–878. Retrieved from https://www.tjnpr.org/index.php/home/article/view/17

Issue

Vol. 6 No. 6 (2022): Tropical Journal of Natural Product Research

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Articles

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